Objective:To explore the method of establishing nonalcoholic fatty liver model in rats in a relatively short period of time,and to observe the pathological changes of the organs in rats.Methods:Twenty-four SD male rats were randomly divided into 3 groups,including the control group(intragastric infusion of saline,10 ml/kg and interperitoneal injection of paraffin oil,3 ml/kg,fed with common diet),CCL4 group(intragastric infusion of saline,10 ml/kg and interperitoneal injection of 40％ CCL4 paraffin oil,3 ml/kg,fed with common diet),and experimental group(intragastric infusion of high fat diet and high cholesterol food,10 ml/kg and interperitoneal injection of by 40％,3 ml/kg,fed with common diet).These treatments were both twice a week.On the third weekend,all rats were killed to observe the pathological changes of the organs including the liver,cardiac,kidney and spleen by HE dyeing.Results:High fat and high cholesterol food can cause obvious fatty degeneration in liver cells within 21 days,but it has little effect on other organs.Conclusion:The method that high fat and cholesterol food lavage can build the nonalcoholic fatty liver disease model in rats in a relatively short of time,greatly shortens the experimental period,has the grate value in the basic experiment.%目的:探索在短时间内快速建立大鼠非酒精性脂肪性肝病模型的方法,并观察造模过程中大鼠各个脏器的病理变化.方法:健康雄性SD大鼠24只,随机分为正常对照组、CCL4组、模型组,每组8只.3组在普通饲料喂养的基础上,正常组给予生理盐水(10 ml/kg)灌胃及石蜡油(3 ml/kg)腹腔注射,CCL4组给予生理盐水(10 ml/kg)灌胃及40％ CCL4石蜡油溶液(3 ml/kg)腹腔注射,模型组给予高脂高胆固醇食物(10 ml/kg)灌胃及40％ CCL4石蜡油(3 ml/kg)腹腔注射,均为2次/周.造模第3周后处死大鼠.HE染色法观察造模过程中大鼠肝脏、肾脏、心脏及脾脏的改变.结果:高脂高胆固醇食物灌胃能在21 d内引起肝脏细胞发生明显的脂肪变性,但对其他组织器官的影响不大.结论:高脂高胆固醇食物灌胃的方法能在较短时间内构建大鼠非酒精性脂肪性肝病的模型,大大缩短了实验周期,具有一定的基础实验推广价值.
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