雷尼酸锶对尾悬吊大鼠骨组织形态计量学的影响

摘要

目的 观察雷尼酸锶(SR)对尾悬吊大鼠骨组织形态计量学的影响.方法 选用30只3月龄SPF级雄性SD大鼠,随机分为全程对照组(n=6)、尾悬吊时服用SR组(n=6)、尾悬吊后服用SR组(n=6)、全程服用SR组(n=6)和尾悬吊后对照组(n=6).8周(其中尾悬吊4周)后处死,取胫骨上端松质骨进行骨组织形态计量学观察.结果 与全程对照组比较,SR干预组静态参数[骨小梁面积百分数(%Tb.Ar)、骨小梁厚度(Tb.Th)、骨小粱数目(Th.N)、骨小梁分离度(Tb.Sp)]、骨形成参数[荧光标记周长百分率(%L.Pm)、单位骨小梁面积骨形成率(BFR/TV)、矿化沉积率(MAR)]及骨吸收参数单位骨小梁面积上的成骨细胞数(Oc.N)均无统计学差异.与尾悬吊后对照组比较,SR干预组Tb.Th、Th.N、%Tb.Ar增加,Tb.Sp减少,提示骨量增加,%L.Pm、BFR/TV、MAR等骨形成参数增加,骨吸收参数Oc.N降低,而SR干预各组间静态参数、骨形成参数及骨吸收参数均无统计学差异.结论 SR可明显改善尾悬吊大鼠骨组织形态计量学指标,对失重性骨质疏松有防治作用.%Objective To investigate the effect of strontium ranelate (SR) on the bone histomorphometry of tail-suspended rat.Methods Thirty male SD rats (3 months old) were randomly divided into five groups (6 each): rats in SR-1 group underwent tail suspension for 4 weeks and given SR simultaneously. In SR-2 group rats received tail suspension for 4 weeks and given SR thereafter. In SR-3 group rats underwent tail suspension for 4 weeks and given SR during the whole course. In control-1 group rats underwent tail suspension for 4 weeks and not given SR, and in control-2 group rats did not undergo tail suspension and were not given SR. Animals were sacrificed 8 weeks (including 4 weeks of tail suspension) later, and the cancellous bone of upper end of tibia was then analyzed histomorphologically. Results There were no significant differences between control-2 group and the 3 SR groups in static parameters [trabecular bone area ( ％Tb. Ar) , trabecular thickness (Tb. Th) , trabecular number (Th. N) and trabecular separation (Tb. Sp)] and dynamic parameters [labeled perimeter ( ％ L. Pm) , bone formation rate (BFR/TV) , mineral apposition rate (MAR), and osteoclast number (Oc. N)]. On then other hand, Tb. Th, Th. N, ％Tb. Ar, ％L. Pm, BFR/TV and MAR increased, and Tb. Sp and Oc. N decreased in the 3 SR groups compared with those in control-1 group, implying that the bone mass was increased. No significant difference was found in all the parameters mentioned above between the 3 SR groups. Conclusions Strontium ranelate may obviously improve the bone histomorphometry of tail suspended rats, and have an effect on preventing osteoporosis caused by agravity.