人源高致病性H5N1不同蚀斑特性病毒致病力研究

摘要

Objective To determine the pathogenicity of human high pathogenic H5N1 virus with different plaque property, and proide a new train of thought and basis for research on crossing species-genus transmission of aian influenza virus. Methods Variants with different plaque property (larger- and smaller-plaque variant) were isolated from A/ Beijing/01/03 (H5N1) ( BJO1) by the plaque purification. Mice were inoculated intranasally (i. n. ) with each kind of isolated variant and monitored for 14 days to record and calculate the body weight change and the survival rate. The difference in pathogenicity of isolated variants was estimated with proper principle of statistics. Results The plaques of original strain of BJO1 virus were heterogeneous with various size and shape. Larger-plaque variants (L1 and L2) and smaller-plaque variants (S1,S2 and S3) were isolated separately. The pathogenicity was higher in S1 and S2 variants than in S3, L1 and L2 variants, and that of L2 variant was the lowest. Variants forming different plaques were heterogeneous in pathogenicity,and those forming same plaques were also heterogeneous in pathogenicity. Conclusion There is no linear correlation between the pathogenicity of variants and the size of plaque they formed. The variants with different pathogenicity could be isolated from the same H5N1 virus strain by plaque purification. These results would proide a basis for vaccine development and the studies on molecular mechanism of H5N1 virus.%目的 分析不同空斑特性病毒致病力的差异,为禽流感病毒跨种属传播机制研究提供新的思路与依据.方法 利用病毒蚀斑技术,从A/Beijing/01/03(H5N1)(BJ01)病毒中分离纯化不同蚀斑特性的病毒--较大和较小蚀斑病毒.采用纯化的大、小斑病毒滴鼻感染小鼠,观察并记录感染后14d小鼠体重和死亡数变化,分析大、小斑病毒致病力的差异.结果 BJ01 株原始病毒呈现大小不一的混合斑状态,从 BJ01株分离纯化了大斑病毒L1、L2和小斑病毒S1、S2、S3,其中小斑病毒S1和S2的致病力较强,大斑病毒L1和小斑病毒S3的致病力较弱,大斑病毒L2病毒致病力最弱.同种空斑特性的病毒致病力差异显著,不同空斑特性病毒在致病力上也存在显著差异.结论 H5N1病毒的空斑大小与病毒致病力没有线性相关性,但可以根据空斑大小,利用蚀斑技术分离纯化出致病力差异明显的病毒.