目的：观察红景天苷对人HaCaT细胞转录因子Nrf2核转位及HaCaT细胞受紫外线照射后谷胱甘肽和丙二醛的影响。方法将HaCaT细胞分为5组，第1组未经任何处理（G1），第2组接受100 mJ/cm2剂量紫外线照射（G2），第3组、第4组及第5组分别采用20、40μg/ml及80μg/ml红景天苷（G3、G4、G5）培养24 h后，再采用100 mJ/cm2剂量紫外线照射，免疫荧光法观察HaCaT细胞Nrf2核转位情况，分光光度法分别检测细胞丙二醛（MDA）、谷胱甘肽（GSH）水平。结果20、40、80µg/ml红景天苷均能够诱导紫外线照射细胞后Nrf2发生核转位，其中40µg/ml和80µg/ml的红景天苷作用尤为显著。G2 MDA较G1显著升高(P＜0.01)，G4、G5 MDA较G2显著降低(均P＜0.05)，G2 GSH较G1显著降低(P＜0.005)，G4、G5 GSH较G2显著升高(均P＜0.01)。结论红景天苷可能通过促进紫外线照射HaCaT细胞的Nrf2核转位，降低MDA及升高GSH的水平，从而减轻紫外线照射对细胞的氧化损伤。%Objective To investigate the effect of salidroside on Ultraviolet B(UVB)-irradiated HaCaT cells by upregulating the activity of Nrf2. Observe the change of MDA and GSH. Methods Cells were divided into five groups: the cells of G1 untreated, the cells of G2 given UVB irradiation with 100 mJ/cm2; the cells of G3, G4 and G5 added with 20, 40μg/ml and 80μg/ml salidroside respectively 24 hours before given UVB irradiation with 100 mJ/cm2. 24 hours later the HaCaT cells were analyzed by immunocytochemistry to observe Nrf2 nuclear translocation. The Levels of MDA and GSH were measured by spectrophotometric method. Results Salidroside, especially of 40μg/ml and 80 μg/ml salidroside, resulted in Nrf2 nuclear translocation significantly. Compared with G1, the levels of MDA increased significantly(P<0.01) and the levels of GSH decreases significantly in G2 (P<0.005). However, the levels of MDA in 40μg/ml and 80μg/ml salidroside treated group decreased significantly compared with G2 (all P<0.05). The levels of GSH in 40μg/ml and 80μg/ml salidroside treated group increased significantly compared with G2 (all P<0.01). Conclusion Salidroside may reduce the oxidative damage by UV radiation in HaCaT cells through enhanced Nrf2 nuclear translocation, reduced the level of MDA and raised the level of GSH.
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