狼毒大戟石油醚提取物和乙酸乙酯提取物的半数致死量测定

摘要

目的：研究狼毒大戟石油醚提取物和乙酸乙酯提取物对小鼠的半数致死量(LD50).方法：150只昆明种小鼠，完全随机平均分组，每组10只.腹腔注射给药，分别给予不同浓度的狼毒大戟石油醚提取物和乙酸乙酯提取物，以小鼠急性死亡率为指标，求狼毒大戟石油醚提取物和乙酸乙酯提取物的半数致死量(LD50)和 LD50的95%可信区间.结果：狼毒大戟石油醚提取物和乙酸乙酯提取物对小鼠的半数致死量(LD50)分别为31.03 mg/kg、1538.58 mg/kg.结论：狼毒大戟石油醚和乙酸乙酯提取物具有一定的毒性，实验结果为进一步研究和开发狼毒大戟提供了毒性依据.%Objective:To measure the half lethal dose(LD50)of petroleum ether extract and ethyl acetate extract of Euphorbia fischeriana Steud in mice.Method:Totally 150 kunming mice in half genders were randomly divided into corresponding groups,each had 10 mice.Intraperitoneal injection the mice with extractive of Euphorbia fischeriana Steud and observe the toxic symptom,and the LD50 and 95% confidence interval of the drug was measured with acute mortality rate as index.Result:The half lethal dose(LD50)of petroleum ether extract and ethyl acetate extract of Euphorbia fischeriana Steud in mice were 31.03 mg/kg,1538.58 mg/kg.Conclusion:The petroleum ether extract and ethyl acetate extract of Euphorbia fischeriana Steud have some toxicity.The experimental result provided toxicity data for further research and development.