2014-2015年南京市新发现HIV/AIDS病例首次T淋巴细胞亚群的检测分析

摘要

Objective:To examine the level of immunity and disease progression through the first detection of T lymphocyte subset count test of newly detected HIV/AIDS cases in Nanjing 2014-2015.Method:A total of 610 whole blood samples collected from HIV/AIDS cases were stained with four color fluorescence monoclonal antibody, and detected the absolute number of T lymphocyte subset by the single platform flow cytometry.The results were statistically analyzed by Excel and SPSS software after counting the ratio of CD4+/CD8+.Result:The mean value of absolute number of CD3+T was (1731±823)/μL,CD4+T was (384±231)/μL,CD8+T was (1268±665)/μL and CD4+/CD8+ was (0.33±0.20).And the persons less than 200/μL(CD4+ T-LC) and 1 (CD4+/CD8+) account for 21.3% and 99.0% of tested HIV/AIDS cases.Further analysis showed that the different gender,age and transmission had influence on T lymphocyte subsets test result.Conclusion:The level of CD4+T lymphocytes in the first detection among HIV/AIDS patients is low in Nanjing area,21.3% of newly detected HIV/AIDS patients have already been AIDS,early intervention and early treatment should be strengthened to assure the first time of CD4 count test,which is important for prevention and control of AIDS.%目的：通过对南京市2014-2015年新发现艾滋病病毒感染者/艾滋病患者（HIV/AIDS）病例首次外周血T淋巴细胞亚群的检测，了解并估计病例的免疫状况及疾病进展情况。方法：以四色荧光抗体标记610份新发现HIV/AIDS病例的抗凝全血，采用单平台流式细胞仪检测T淋巴细胞各亚群的绝对值，计算CD4+/CD8+T淋巴细胞的比值。所得数据用Excel和SPSS软件进行统计分析。结果：T淋巴细胞亚群检测结果均值：CD3+T为（1731±823）个/μL，CD4+T为（384±231）个/μL，CD8+T为（1268±665）个/μL，CD4+/CD8+为（0.33±0.20）。21.3%病例CD4+T<200个/μL，CD4+/CD8+<1的病例占99.0%。进一步分析发现，不同性别、年龄和传播途径对T淋巴细胞亚群计数均有影响。结论：南京市HIV/AIDS病例首次CD4+T淋巴细胞水平较低，21.3%新发现病例已进入艾滋病期，应进一步加大早发现早治疗的工作力度，保证新发现病例能够及时检测CD4细胞数量，把握最佳治疗时机。