急性心肌梗死患者内脏素mRNA基因表达及临床相关性的研究

摘要

Objective:To investigate the expression of mRNA gene in the adipose tissue of patients with acute myocardial infarction,and to provide a new method for the prevention and treatment of patients with acute myocardial infarction.Method:According to the criteria of clinical diagnosis,80 patients with acute myocardial infarction were enrolled as acute myocardial infarction group, and 100 normal people were served as control group. RT-PCR method was used to detect the level of mRNA and the expression level of gene,and the clinical data of the two groups were collected and compared.Result:The plasma visfatin level and the quantitaitive of visfatin mRNA expression adipose tissues of acute myocardial infarction of patients were significantly higher than those in control group(P<0.05).The expression of mRNA in adipose tissue of patients with acute myocardial infarction was positively correlated with WHR,TG,TC(P<0.05). The amount of internal organs in patients with acute myocardial infarction was positively correlated with the clinical TG,TC,and LDL-C(P<0.05).Conclusion:In patients with acute myocardial infarction, the increased expression of the internal organs and the concentration of the internal organs may be an independent risk factor for acute myocardial infarction.%目的：探讨急性心肌梗死患者内脏素基因在脂肪组织中表达情况，以及内脏素mRNA基因表达与患者临床相关性研究，为急性心肌梗死患者防治提供新的方法。方法：根据临床诊断标准将80例急性心肌梗死患者作为急性心肌梗死组，将100例正常人作为对照组。用RT-PCR方法检测内脏素mRNA的水平和基因的表达水平，并同时收集比较两组的临床检查数据指标。结果：急性心肌梗死患者组的血清内脏素浓度及脂肪组织中的内脏素mRNA基因表达均高于对照组（P<0.05）。急性心肌梗死患者脂肪组织的mRNA的表达量与WHR、胆固醇、甘油三酯均呈正相关性（P<0.05）。急性心肌梗死患者内脏素的量与机体临床的甘油三酯、胆固醇、LDL-C均呈正相关性（P<0.05）。结论：急性心肌梗死患者中内脏素基因与浓度表达增高，可能是急性心肌梗死的一个独立危险因素。