阻塞性黄疸是一种临床常见的肝胆疾病,目前可通过外科手术解除阻塞,但已造成的肝损伤很难修复.虎纹克胰素(huwentoxin-Ⅺ,HWTX-Ⅺ)是一种具有高效胰蛋白酶抑制活性的多肽,其对肝损伤的保护作用尚未进行实验性研究.有报道称,有氧运动能减轻炎症以及促进肝再生和修复.为了探讨HWTX-Ⅺ给药配合有氧运动对阻塞性黄疸引起的小鼠肝损伤的拮抗作用,采用胆总管悬挂于腹壁的造模,分别以跑台和尾静脉输注HWTX-Ⅺ为干预手段,使用HE染色观察阻塞性黄疸小鼠肝组织形态结构变化,应用RT-PCR和免疫组化技术检测相关炎性细胞因子的基因和蛋白质表达水平.结果显示,HWTX-Ⅺ给药配合有氧运动有利于胆管阻塞的小鼠肝损伤的修复,而且这种影响的机制可能与NF-κB通道因子的表达下调有关.同时,HWTX-Ⅺ与有氧运动的结合干预对以上模型的干预效果较二者单因素干预的效果更佳.%Obstructive jaundice (OJ) is a common clinical disease.Although the obstruction could be removed by surgery,the resulting liver damage is difficult to repair.Huwentoxin-Ⅺ (HWTX-Ⅺ) is an effective trypsin inhibitor,but its potential effect on liver injury has not been explored experimentally.On the other hand,aerobic exercise has been reported to be effective in the amelioration of inflammation,and to promote the process of liver regeneration.In order to investigate the antagonistic effect of HWTX-Ⅺ treatment combined with aerobic exercise on liver injury caused by obstructive jaundice,a mouse model with the common bile duct hanging at the abdominal wall was established.In addition,treadmill training was adopted for aerobic exercise and HWTX-Ⅺ was administrated by tail vein injection.After the intervention,the morphological changes in liver were observed by HE staining and the expression levels of the inflammatory-related molecules were detected by RT-PCR and immunohistochemistry.The results showed that HWTX-Ⅺ administration combined with aerobic exercise could be beneficial to the recovery of damaged mouse liver induced by biliary obstructive jaundice.The underlying mechanism could be involved in the down-regulation of factors in the NF-κB signaling.Moreover,the joint effect of HWTX-Ⅺ treatment and aerobic exercise was much more efficient than any single-factor intervention.
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