反相高效液相色谱法测定虎杖根中白藜芦醇及其苷的含量

摘要

建立反相高效液相色谱法同时测定不同根龄虎杖根中虎杖苷和白藜芦醇含量。采用KromasilC18（4.6mm×250mm,5μm）色谱柱,流动相为甲醇-1.0%磷酸水溶液（50：50,v/v）,流速0.8mL/min,检测波长319nm,柱温为30℃。虎杖苷和白藜芦醇的进样量分别在0.716~7.160μg（r=0.9996）和0.740~7.400μg（r=0.9998）范围内与峰面积呈良好的线性关系;平均回收率（n=6）分别为99.7%和98.5%。本方法快速简便,重复性好,专属性强,可作为虎杖根的质量%A reserved-phase HPLC method for the content determination of erbascoside and resveratrol in root of Polygonum cuspidatum was established. The analytical was carried out with Kromasil C18 （4.6 mm×250 mm, 5 μm） column and the mobile phase consisted of acet