深色有隔内生真菌对樟子松苗木生长及抗立枯病的影响

摘要

The dark septate endophyte(DSE)fungi is main rhizosphere microorganism groups of coniferous species,and play an im-portant role in growth promote and stress resistance to host.Seedling biomass,physiological index of disease resistance and the inci-dence of damping off were measured to research the growth promotion and disease resistant of Pinus sylvestris var. Mongolica inoculat-ed by dark septate endophyte fungi D340. The results indicated that:compared with the control,the seedling height,ground diameter, fresh and dry weight were increased 9.15%,5.81%,12.9%and 23.23%respectively.The seedling CAT,POD and SOD enzyme of Pinus sylvestris var. Mongolica inoculated by dark septate endophyte fungi D340 were increased 26.22%,22.74%and 7.31%,the MDA content was decreased 19.51%,and theβ-1,3-glucanase and chitinase activity were increased 27.85%and 8.69%in contrast to control.%深色有隔内生真菌是针叶树种根际微生物的主要类群，在与宿主植物互作过程中可发挥促生抗逆的作用，该文通过测定樟子松苗木生物量、抗逆生理指标及立枯病发生率，探讨深色有隔内生真菌D340 Phialocephala helvetica接种后对樟子松生长及抗病性的影响。结果表明：苗高、地径、鲜重和干重比对照分别提高9.15%、5.81%、12.9%和23.23%。立枯丝核菌诱导处理后，接种深色有隔内生真菌的樟子松苗木保护酶CAT、POD和SOD酶活性分别比对照提高26.22%，22.74%和7.31%，丙二醛含量降低19.51%，β-1,3-葡聚糖酶和几丁质酶活性分别提高27.85%和8.69%。