C型凝集素LSECtin识别细菌的研究

摘要

目的:通过sLSECtin-Fc蛋白与细菌及细胞的黏附,寻找能与LSECtin结合的细菌.方法:通过ELISA检测sLSECtin-Fc与细菌的黏附,同时构建CHO-pDsRed1-N1-LSECtin稳定细胞株进行与细菌的黏附实验,荧光显微镜进行镜鉴拍照.结果:获得能够与sLSECtin-Fc蛋白黏附的细菌,并且通过过表达LSECtin的稳定株与细菌的黏附,发现LSECtin可以结合大肠杆菌和肺炎链球菌,而不能结合金黄色葡萄球菌和肺炎克雷伯杆菌.结论:发现LSECtin可以结合细菌,为进一步研究LSECtin在先天免疫中的作用奠定了基础.%Objective: To investigate whether liver and lymph node sinusoidal endothelial cells lectin(LSECtin)recognizes bacteria by the adhesion assay. Methods: Detecting the adhesion of bacteria to recombinant sLSECtin-Fc protein by ELISA assay, and to the membrane-bound LSECtin stably expressed on the surface of CHO cells by fluorescence microscope assay. Results: Both recombinant sLSECtin-Fc protein and the membrane-bound LSECtin were able to recognize E. coli and Streptococcus pneumoniae, but can not recognize S. aureus and Klebsiella pneumoniae. Conclusion: We showed that LSECtin could recognize bacteria, which would provide novel insights into the role of LSECtin in the innate immunity for further study.