NAD(P)H氧化酶p22phox亚基C242T基因多态性与脑梗死的关系

摘要

目的 了解上海地区汉族脑梗死患者烟酰胺腺嘌呤二核苷酸磷酸氧化酶[NAD(P)H氧化酶]p22phox亚基C242T基因多态性和等位基因频率的分布情况,探讨其多态性与脑梗死的关系.方法 采用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP),对176例脑梗死患者和131名健康老年人的C242T位点进行基因分型,计算等位基因的频率分布.结果 脑梗死患者血清三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、血糖浓度和血压与老年健康对照组之间差异具有统计学意义(P<0.05).脑梗死组与老年健康对照组的CC、CT、TT基因型频率分别为92.05%、7.95%、0与93.89%、5.34%、0.76%;C、T等位基因频率分别为96.02%、3.98%与96.56%、3.44%,2组间的差异均无统计学意义(P>0.05).结论 p22phox亚基C242T位点的单核苷酸多态性与脑梗死的发生可能无关.%Objective To investigate the distribution of the gene polymorphism and allele frequency of the NAD(P) H oxidase p22phox C242T, and evaluate the relationship between the polymorphism and cerebral infarction among Han population in Shanghai. Methods The gene C242T was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique in 176 cerebral infarction patients and 131 healthy elders. The frequency distribution of allele was calculated. Results The serum triglyeride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), blood glucose level and blood pressure in cerebral infarction patients were much higher than those in the healthy elders ( P ＜ 0.05 ). However, the genotype distributions of the cerebral infarction patients were 92.05％ (CC), 7.95％ (CT) and 0(TT). The genotype distributions of the healthy elders were 93.89％ (CC), 5.34％ (CT) and 0.76％ (TT). The allele frequency in the cerebral infarction patients was distributed as 96.02％ (C) and 3.98％ (T), while that in the healthy elders was distributed as 96.56％(C) and 3.44％ (T). There was no significant difference in genotype and allele frequency distribution between the cerebral infarction patients and the healthy elders(P＞0.05). Conclusions The NAD(P)H oxidase p22phox C242T polymorphism might not associate with cerebral infarction.