2型糖尿病患者CR1基因多态性与红细胞CR1数量、黏附活性相关性研究

摘要

Objective To study on the correlation of complement receptor type 1 ( CR1) gene polymorphism with erythrocyte CR1 expression and its adhesive activity in patients with type 2 diabetes mellitus (T2DM). Methods A total of 132 patients with T2DM were determined for the CR1 gene polymorphism by restriction incision enzyme Hind III polymerase chain reaction restriction fragment length polymorphism ( PCR-RFLP) ,and enzyme -linked immunosorbent assay (ELISA) was performed to detect the expression of CR1 on erythrocyte and its adhesive activity . Meanwhile,124 healthy subjects were enrolled as controls. The results were analyzed statistically. Results The expression of CR 1 on erythrocyte and its adhesive activity in patients with T 2DM were lower than those in healthy controls ( P < 0. 01). The CR genotype distribution frequencies from high to low were type HH ,type HL and type LL by turns. The allele L frequency of CR gene in T2DM patients (14. 8% ) was higher than that in healthy controls (11.3%),and there was no statistical significance in CR1 genotype frequency and allele frequency between the 2 groups (P > 0. 05 ). The expression of CR1 and adhesive activity of type HH and type HL had no statistical significance (P > 0. 05). The expression of CR1 and adhesive activity of type HH and type HL in T2DM patients were significantly lower than those in healthy controls (P < 0. 05). Conclusions The genotype and allele frequencies in patients with T2DM have no statistical difference with those in healthy controls ,but the expression of CR 1 on erythrocyte and the adhesive activity in patients with T2DM decrease significantly. It indicates that the decrease of erythrocyte CR 1 adhesive activity may be attribute to the occurrence of T2DM.%目的 研究2型糖尿病(T2DM)患者1型补体受体(CR1)基因多态性与红细胞CR1数量、黏附活性的相关性.方法 应用限制性内切酶Hind Ⅲ聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术测定132例T2DM患者和124名正常健康人群CR1基因多态性,用酶联免疫吸附试验(ELISA)测定红细胞的CR1数量,检测红细胞黏附活性,并做统计学分析.结果 T2DM患者红细胞表面的CR1数量和黏附活性均低于正常对照组(P<0.01).CR1基因型在2组的分布频率均为HH型最高、HL型次之、LL型最低.T2DM组CR1基因等位基因L频率为14.8%,高于对照组(11.3%),但2组CR1基因型频率和等位基因频率差异均无统计学意义(P>0.05).2组组内比较,HH型与HL型红细胞CR1数量及黏附活性差异均无统计学意义(Ρ均>0.05).T2DM组HH型与HL型红细胞CR1数量与黏附活性均低于正常对照组(Ρ<0.05).结论 T2DM患者与正常对照者间CR1基因型频率和等位基因频率无差异,而T2DM患者红细胞CR1数量及黏附活性减低,提示T2DM的发生与红细胞免疫功能低下有关.