Objective To investigate the clinical significance of serum tumor necrosis factor-alpha (TNF-α), interleukin 12(IL-12),interleukin 4 (IL-4),immunoglobulin (Ig)G,IgA,IgM,complement (C)3,C4 and C-reactive protein(CRP)in the diagnosis of pregnancy induced hypertension syndrome and the change regularity in different degrees of pregnancy induced hypertension syndrome.Methods The serum samples were collected from 1 14 patients with pregnancy induced hypertension syndrome and 93 healthy pregnant women.The levels of TNF-α,IL-12 and IL-4 were determined by enzyme-linked immunosorbent assay(ELISA),the levels of IgG,IgA,IgM,C3,C4 and CRP were determined by immunonephelometry.Results The levels of serum TNF-α,IL-12 and CRP in pregnancy induced hypertension sydrome group were higher significantly than those in healthy pregnant group (P<0.001 ).The levels of serum IL-4,IgG,IgM,C3 and C4 in pregnancy induced hypertension syndrome group were lower than those in healthy pregnant group (P <0.001 ).The levels of serum TNF-α,IL-12 and CRP in prophase of heavy degree eclampsia were higher significantly than in prophase of light degree eclampsia (P<0.05,0.001 and 0.001),and the levels of serum IL-4,IgG,C3 and C4 were lower (P<0.05).There was a positive correlation of serum CRP with IL-12 and TNF-αamong pregnancy induced hypertension syndrome,prophase of light degree eclampsia,prophase of heavy degree eclampsia and healthy pregnant groups (P<0.05 ).There was a negative correlation of serum TNF-αwith C3 and C4 among pregnancy induced hepertension sydrome,prophase of light degree eclampsia,prophase of heavy degree eclampsia and healthy pregnant groups,respectively (P<0.05 ).Conclusions The levels of serum TNF-α,IL-12, IL-4,IgG,IgM,C3,C4 and CRP are different-degree abnormal in pregnancy induced hypertension syndrome group, and the levels of serum IL-12,IL-4,IgG,C3,C4 and CRP reflect the severity degree of pregnancy induced hypertension syndrome,and can be effective markers for judging patient′s conditions.%目的：探讨血清肿瘤坏死因子（TNF-α）、白细胞介素12（IL-12）、白细胞介素4（IL-4）、免疫球蛋白（Ig）G、IgA、IgM，补体C3、补体C4、C反应蛋白（CRP）在不同程度妊娠高血压综合征（简称妊高征）中的变化规律及其临床应用价值。方法采用酶联免疫吸附试验（ELISA）检测114例妊高征患者（其中轻度子痫前期39例、重度子痫前期75例）和93例正常晚期妊娠妇女血清TNF-α、IL-12和IL-4水平，采用免疫比浊法检测IgG、IgA、IgM、补体C3、补体C4和CRP。结果妊高征组TNF-α、IL-12和CRP明显高于正常晚期妊娠组（P均＜0．001），IL-4、IgG、IgM、补体C3和补体C4明显低于正常晚期妊娠组（P均＜0．001）。重度子痫前期组TNF-α、IL-12和CRP明显高于轻度子痫前期组（P＜0．05、P＜0．001、P＜0．001），IL-4、IgG、补体C3和补体C4明显低于轻度子痫前期组（P均＜0．05）。妊高征组、轻度子痫前期组、重度子痫前期组、正常晚期妊娠组血清CRP与IL-12、TNF-α呈正相关（P＜0．05），血清TNF-α与补体C3、补体C4呈负相关（P＜0．05）。结论妊高征患者血清TNF-α、IL-12、IL-4、IgG、IgM、补体C3、补体C4和CRP出现不同程度的异常。IL-12、IL-4、IgG、补体C3、补体C4和CRP可反映妊高征的严重程度，可作为判断病情的指标。
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