K562细胞ssDNA适配子检测方法对慢性粒细胞白血病诊断价值的研究

摘要

Objective To evaluate the significance of K562 cell ssDNA aptamer detection method for the clinical diagnosis of chronic myeloid leukemia(CML).Methods A total of 5 mL blood samples of 18 patients with CML,22 patients with other leukemia and 20 healthy subjects were collected.The granulocytes were separated,and aptamer detection method was established for the determination of fluorescence intensity.The receiver operating characteristics (ROC)curve was drawn.Results The fluorescence intensities were 492.26 ±41.67 in CML group,466.86 ±33.23 in other leukemia group and 469.33 ±37.13 in healthy control group,and the results showed no statistical significance (P=0.078 and P=0.243).There was no statistical significance between other leukemia group and healthy control group (P=0.835).The area under ROC curve was 0.702,the optimal positive threshold was 475.13,the sensitivity was 83.33%,the specificity was 54.76%,the positive predictive value was 44.11%,and the negative predictive value was 88.46%.Conclusions The aptamer detection method has a low accuracy in the clinical diagnosis of CML,and it has some limitations for clinical application.%目的：评价K562细胞ssDNA适配子检测方法（简称适配子检测法）对慢性粒细胞白血病（CML）的临床诊断价值。方法采集18例CML患者、22例其他白血病患者、20名健康体检者（正常对照组）的血液标本各5 mL，分离粒细胞，用建立的适配子检测法检测荧光强度，绘制受试者工作特征（ROC）曲线。结果 CML组荧光强度为492．26±41．67，与其它白血病组（466．86±33．23）及正常对照组（469．33±37．13）比较，差异无统计学意义（P值分别为0．078、0．243）；其它白血病组和正常对照组比较，差异亦无统计学意义（P＝0．835）。适配子检测法诊断CML的ROC曲线下面积为0．702，最佳阳性临界值为475．13，灵敏度为83．33％、特异性为54．76％、阳性预测值为44．11％、阴性预测值为88．46％。结论适配子检测法对CML的诊断具有较低的准确性，实际应用还有一定的局限性。