抗核抗体与EB病毒抗体水平的关系

摘要

ObjectiveTo evaluate the specific antibody immune response against Epstein-Barr virus(EBV) in patients with anti-nuclear antibody(ANA) positivity and negativity.MethodsANA was determined by indirect immunofluorescence. Anti-extractable nuclear antigen(ENA) antibodies were determined by immunoblotting. Serum EBV-virus capsid antigen(VCA) IgM,EBV-VCA IgG,EBV-early antigen(EA) IgG and EBV nuclear antigen(EBNA-1) IgG levels were determined by enzyme-linked immunosorbent assay(ELISA) in 80 patients with ANA positivity and 48 healthy subjects. The difference of EBV antibody positive rates and levels was evaluated in ANA-positive patients in comparison with those in ANA-negative healthy controls. The relationship between EBV antibody level and the types and numbers of ENA antibodies was analyzed.ResultsEBV-VCA IgG,EBV-EA IgG and EBNA-1 IgG levels were higher in ANA-positive patients than those in healthy controls(P<0.01),and ANA-positive patients showed a higher EBV-EA IgG positive rate compared to healthy controls(χ2=5.747,P=0.017). Furthermore,elevated EBNA-1 IgG was found in anti-SS-A/Ro antibody positive and anti-SS-B/La antibody positive groups compared to those in anti-SS-A/Ro antibody negative and anti-SS-B/La antibody negative groups (Z=-2.630,P=0.008;Z=-2.515,P=0.011). EBV-VCA IgG and EBV-EA IgG levels had no statistical significance (P>0.05). EBV-VCA IgG and EBV-EA IgG were higher in anti-nucleosome antibody positive group than those in anti-nucleosome antibody negative group(Z=-2.355,P=0.018;Z=-2.017,P=0.043),while there was no statistical significance for EBNA-1 IgG(P>0.05). There was difference in the number of anti-ENA antibodies and the distribution of EBV-EA IgG levels(χ2=11.380,P=0.023).ConclusionsEBV reactivation might be correlated with the presence of ANA.%目的 探讨EB病毒(EBV)特异性抗体对抗核抗体(ANA)阳性患者和ANA阴性健康对照者的免疫反应.方法 采用间接免疫荧光法检测ANA,采用免疫印迹法检测抗可提取性核抗原(ENA)抗体,采用酶联免疫吸附试验(ELISA)检测80例ANA阳性患者和48名体检健康者血清EBV-衣壳抗原(VCA)IgM、EBV-VCA IgG、EBV-早期抗原(EA)IgG和EBV核抗原(EBNA-1)IgG水平,比较ANA阳性患者与ANA阴性健康对照者的EBV抗体阳性率、EBV抗体水平差异,分析ANA阳性患者EBV抗体水平与ENA抗体种类、个数之间的关系.结果ANA阳性组血清EBV-VCA IgG、EBV-EA IgG和EBNA-1 IgG水平较健康对照组明显升高(P<0.01),且EBV-EA IgG阳性率明显高于健康对照组(χ2=5.747,P=0.017).抗ENA抗体谱中,抗SS-A/Ro抗体、抗SS-B/La抗体阳性组分别与抗SS-A/Ro抗体、抗SS-B/La抗体阴性组比较EBNA-1 IgG水平明显升高(Z=-2.630,P=0.008;Z=-2.515,P=0.011),EBV-VCA IgG和EBV-EA IgG水平差异无统计学意义(P>0.05).抗核小体抗体阳性组与抗核小体抗体阴性组比较EBV-VCA IgG、EBV-EA IgG水平明显升高(Z=-2.355,P=0.018;Z=-2.017,P=0.043),但2组之间EBNA-1 IgG水平差异无统计学意义(P>0.05).抗ENA抗体谱中自身抗体阳性个数不同的ANA阳性患者EBV-EA IgG水平不同(χ2=11.380,P=0.023).结论EBV复发感染在ANA的产生中起重要作用.