不同采血顺序、送检时间及袖带压力对血液流变学的影响

摘要

Objective To investigate the influence of the blood sampling order ,submission time and cuff pres‐sure on hemorheology .Methods 90 individuals conducted healthy physical examination in our hospital from Jun . 2012 to Aug .2013 were randomly divided into three groups ,30 cases in each group .The first group was performed the blood collection for 3 test tubes at the same location ,the order was labelled ,and the blood sample was submitted within 1 h ;4 tubes of blood sample were collected in the second group and submitted at 1 ,4 ,8 ,24 h;the blood pres‐sure cuff was bounded the double arms in the third group ,the cuff pressure were maintained at 40 mm Hg ,110 mm Hg ,then blood sample was collected and sent within 1 h .The blood viscosity and whole blood shear rate of blood samples were compared .Results In the first group ,except the low shear rate ,the blood viscosity and other whole blood shear rate had statistical differences between the samples of second ,third tube and samples of first tube(P<0 .05);the blood viscosity and whole blood shear rate in the second group had statistical differences between the sam‐ples submitted after 6 ,24 h and the samples submitted after 1 h(P<0 .05);the blood viscosity and whole blood shear rate in the third group had statistical differences between the cuff pressure of 40 mm Hg and the cuff pressure of 110 mm Hg (P<0 .05) .Conclusion The blood sampling order ,submission time and sampling cuff pressure could affect the hemorheologic test results .It is suggested that the cuff pressure should not be too high ,the first tube of collected blood sample should be used for the hemorheology detection ,moreover the submission time should be less than 4 h .%目的：探讨采血顺序、送检时间、袖带压力对血液流变学的影响。方法将2012年6月至2013年8月在如东县人民医院行健康查体者90例随机分为3组，各30例。第1组于同一位置采集血液标本3管，注明顺序，1 h内送检；第2组采集4管血液标本，分别于1、4、8、24 h后送检；第3组以血压袖带束缚双臂，将袖带压力分别保持在40、110 mm Hg后采集血液标本，1 h内送检。比较标本的血液黏度、全血切变率。结果除全血低切变率，第1组2、3号管标本的血液黏度及其他全血切变率与1号管比较差异均有统计学意义（P＜0．05）；第2组采血后8、24 h送检标本的血液黏度及全血切变率与采血后1 h送检标本比较差异均有统计学意义（ P＜0．05）；第3组以40 mm Hg压力所采标本的的血液黏度及全血切变率与以110 mm Hg压力所采标本比较差异有统计学意义（P＜0．05）。结论血液标本的采样顺序、送检时间及采样时袖带压力均可影响血液流变学的检测结果，建议采血时袖带的压力不宜过大，将采集的第1管标本用以血液流变学检测，且以采血后4h以内送检为宜。