目的 制备抗树鼩、长爪沙鼠和灰仓鼠IgG抗体,并分别用异硫氰酸荧光素和辣根过氧化物酶进行标记.方法 采用Protein G分别提纯树鼩、长爪沙鼠和灰仓鼠血清中的IgG,纯化后免疫家兔,制备兔抗三种动物IgG抗体；采用亲和层析纯化兔抗三种动物IgG抗体,并分别进行FITC和HRP标记.结果 分别获得抗灰仓鼠、树鼩和长爪沙鼠IgG抗体,浓度分别为:2.2 mg/ml、2.3 mg/ml和2.3 mg/ml; FITC标记的兔抗灰仓鼠、树鼩和长爪沙鼠IgG含量分别为:1.19 mg/ml,1.36 mg/ml和1.55 mg/ml; HRP标记的兔抗灰仓鼠、树鼩和长爪沙鼠IgG含量分别为:0.84 mg/ml、1.09 mg/ml和1.31 mg/ml.结论 得到了适合检测用的FITC和HRP标记二抗,为建立这三种动物致病微生物和寄生虫检测方法提供了试剂.%Objective To prepare anti-IgG of Tupaia belangeri, Cricetulus migratorius and Merionesun guiculatus, and label them by using FITC and HRP. Method The IgG of these three animals were purified using Protein G and the anti-IgG were obtained from immunized rabbits. The anti-IgG were purified through affinity chromatography and labeled by FITC and HRP. Results The anti-IgG concentration of Cricetulus migratorius, Tupaia belangeri and Merionesun guiculatus was 2.2 mg/ml, 2.3 mg/ml and 2.3 mg/ml respectively. The IgG content of FITC labeled anti-IgG for each animal was 1.19 mg/ml, 1.36 mg/ml and 1.55 mg/ml respectively. The IgG content of HRP labeled anti-IgG for each animal was 0.84 mg/ml, 1.09 mg/ml and 1.31mg/ml respectively. Conclusion The study successfully obtained the FITC and HRP labeled anti-IgG, which can be used for the establishment of microorganism detection in these three animals.
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