目的 探讨不同跑台坡度对大鼠运动疲劳指标的影响.方法 32只SD大鼠随机分为对照组以及0°、-15°和15°跑台运动组,运动组大鼠每日按照方案进行跑台训练.大鼠经过6周训练结束后检测血清中葡萄糖(GLU)、尿素(BUN)、乳酸(LA)、肌酸激酶(CK)及血液中血红蛋白(HGB)、红细胞(RBC),腓肠肌指数,对腓肠肌进行HE染色,并统计大鼠被电击次数.结果 与对照组比较,不同跑台坡度对大鼠的血液代谢产物、能量物质及运动能力均有明显变化.其中,各运动组大鼠外周血中GLU、HGB与RBC较对照组显著降低(P＜0.01,P＜0.05),腓肠肌指数显著增高(P＜0.01);15°跑台与-15°跑台运动组血清中LA、BUN浓度及CK活性均显著增高(P＜0.01,P＜0.05).与0°跑台运动组比较,15°跑台与-15°跑台运动组大鼠被电击次数显著增多P＜0.05).HE染色结果显示,运动后各组大鼠腓肠肌细胞有肿胀损伤的趋势.结论 15°跑台坡度比-15°或0°跑台坡度复制大鼠运动疲劳模型较更佳.%Objective To study the influence of exercise fatigue index on different treadmill slope in rats.Methods 32 SD rats were randomly divided into 4 groups:the control group,and 0°-,-15°-and.15 °-treadmill slope exercise groups.The model group was given treadmill exercise according to the scheme.Related indexes were detected after 6 weeks training.The serum content of glucose(GLU),blood urea nitrogen(BUN),lactic acid (LA),creatine kinase (CK),hemoglobin (HGB),red blood cell (RBC).Gastrocnemius muscle index and the number of electric shock in the rats were calculated.Results Compared with the control group,there were significant changes in the blood metabolites,energy material and exercise ability during different treadmill slope exercise in the rats.The GLU,HGB and RBC in the peripheral blood samples of each exercise group were significantly lower than those in the control group (P＜0.01,P＜0.05),and the gastrocnemius muscle index was significantly higher (P＜0.01).The serum of LA,BUN concentration and CK activity were significantly increased (P＜0.01,P＜0.05) in the 15° treadmill and-15° treadmill exercise groups.Compared with the 0° treadmill exercise group,the number of electric shock in the rats were significantly increased in the 15 ° treadmill and-15 ° treadmill exercise groups (P＜0.05).HE staining showed that the gastrocnemius muscle cells of rats had a tendency of swelling and injury after exercise.Conclusions At 15 ° treadmill exercise groups,the exercise fatigue model replication of rats was better than that of 0° or-15 ° treadmill exercise groups.
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