为寻找一种适合于中华猕猴桃（Actinidia chinensisPlanch．）的性别分子标记，以广东省和平县主栽中华猕猴桃的4个雌株品种、4个雄株品种以及“和平红阳”品种种子实生苗为试材，通过基因组DNA提取、RAPD扩增和比较分析，筛选出了6个带型清晰、雌雄性别差异明显的RAPD引物，并从6个引物扩增产物中分离出“和平红阳”种子实生苗雌性特征片段$1044—900和S1044—1350；另外用引物S1032对8个栽培品种雌雄植株基因组DNA扩增后发现了3个与雄性连锁的DNA标记，其中1条片段为NCBIGeneBank收录的猕猴桃雄性链锁标记S1032—850（登陆号：AY336259）．根据NCBI Gene Bank收录的S1032—850序列设计特异性引物对4个雌株品种和4个雄株品种的基因组DNA扩增后发现，此标记在雄株中均有出现，在雌株未出现．%To find a sex-related marker that could be used to identify leaves from 4 cuhivars of male, 4 cuhivars of female and ' Hongyang' the sex of Actinidia chinensis, young seedling kiwifruit plants (Actinidia chinensis) were used to extract their genomic DNA. RAPD amplification was carried out with 20 random primers, from which 6 RAPD primers were selected according to the RAPD differences between male and female plants. Two female-related markers S1044-900 and S1044-1350 were isolated based on their amplification results in all the ' Hongyang' female seedlings,but not in male ones, when the primer S1044 was used in the RAPD amplification. Three male-related markers were found according to the RAPD amplification result with the primer S1032, one of which was the S1032-850 marker included in NCBI database( GenBank accession no.:AY336259). PCR amplification of the DNA samples with a specific primer pair based on S1032-850 sequence showed that the S1032-850 fragment was amplified in all 4 cuhivars male plants, but not in female plants.
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