猪圆环病毒( PCV)两个血清型中只有PCV2为致病性病毒。将ORF2蛋白作为抗原建立区别两型PCV的血清学检测方法。去除N端信号肽的ORF2片段在大肠杆菌中BL21(DE3)中进行了表达。 SDS-PAGE凝胶电泳表明在26 kD处有一个明显的表达条带，并且可以和猪圆环病毒阳性血清发生反应，表明重组蛋白表达成功。以表达的ORF2蛋白作为包被原，建立了检测猪圆环病毒、猪繁殖与呼吸综合征病毒抗体的间接ELISA方法。与商品化试剂盒同时检验78份临床血清，符合率为95%；同时与猪瘟病毒、猪细小病毒、猪伪狂犬病毒均无交叉反应。%Porcine circovirous( PCV) can be sub-classified two serotypes and only PCV2 is pathogenic. ORF2 could be used as special antigen to construct a serum detection method. After deleting the signal sequence, the rest ORF2 sequence was expressed in Escherichia coli BL21(DE3). Result of SDS-PAGE gel electrophoresis indicated a 26 kD stripe, which was consistent with the predicted. Besides, the re-combinant ORF2 protein could react with PCV2 positive serum. ORF2 without signal sequence was suc-cessfully expressed in E. coli. Based on the expressed ORF2 protein, ELISA method was set up for de-tecting PCV2 antibodies. The method presented 95 % coincidence with commercial ELISA kit and no cross-actions with CSFV, PPV, PRV and PRRSV were detected.
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