两种多囊卵巢综合征大鼠模型造模方法的比较

摘要

Airti: To compare two kinds of polycystic ovaries syndrome rat models induced by letrozole and dehydroepiandrosterone. Methods: Rats aged six weeks and 23 days (each 60 cases) were administered letrozole and DHEA respectively to induce PCOS model. Increased weight of rats, oestrous cycle, morphology and histology of ovary, serum hormone levels and the expression of AR.LHR in the ovary were examined. Results; ① The two experimental groups showed less granular cell layer and proliferated mesenchyma in ovary. ②The increased weight, volume,and relative quality of ovaries in letrozole experimental groups were larger than that in control( F = 13. 136,9.778 and 6. 544, P < 0. 05) ;In DHEA group, the volume of ovrian in the experimental group was significantly smaller than in controls( F = 19. 383 ,P < 0. 001 ). ③The level of luteinizing hormone ( LH) and testosterone ( T) of letrozole experimental group were significantly higher than in controls, serum follicle stimulating hormone(FSH) , estradiol ( E2 ) and progesterone ( P) of letrozole experimental group was significantly lower than controls. T and E2 of DHEA experimental group were significantly higher than controls, FSH of DHEA experimental group were significantly lower than controls(P <0. 05). ④The expression of AR.LHR in the ovary of letrozole experimental group were significantly higher than in controls(P <0.05). In DHEA group, the expression of AR in the ovary was significantly higher than in controls(P <0. 05). Conclusion: ①Compared with DHEA groups, PCOS model induced by letrozole in several ways is more similar to the human disease and it is a relative perfect rat model. (2)The ex-pression of AR.LHR in rat ovary can be evaluated indexes for protein level in PCOS model.%目的：采用来曲唑和脱氢表雄酮(DHEA)建立多囊卵巢综合征(PCOS)大鼠模型，并对2种模型进行比较。方法：6周龄和23日龄SD雌性大鼠各60只分别用于来曲唑和DHEA建模，均随机分为实验组、溶剂对照组和空白对照组各20只；从建模前后大鼠体质量增长、动情周期、卵巢变化、血清性激素水平、卵巢中雄激素受体(AR)和黄体生成素受体(LHR)等指标进行模型评价。结果：①2种造模方法的实验组大鼠卵巢颗粒细胞层明显减少，间质增生。②来曲唑造模的实验组实验前后体质量增加量、卵巢体积和相对质量均大于对照组(F=13.136F9.778和6.544，P均＜0.05)；DHEA造模的实验组仪卵巢体积小于对照组(F=19.383，P＜0.001)。③来曲唑造模的实验组黄体生成素、睾酮(T)含量高于对照组，而卵泡刺激素(FSH)、雌二醇(E2)、孕酮(P)低于对照组(P＜0.05)；DHEA造模组的实验组血清T、E2高于对照组,FSH低于对照组(P＜0.05)。④来曲唑造模的实验组AR、LHR含量均高于对照组(P＜0.05)；DHEA造模的实验组仅AR高于对照组(P＜0.05)。结论：①来曲唑大鼠模型更接近于人PCOS特征，是相对理想的PCOS模型。②AR、LHR参与了多囊状卵巢的形成，可作为PCOS模型蛋白质水平的评价指标。