睾酮受体蛋白表达水平对高血压大鼠血管重构的影响

摘要

Aim:To explore the effect of the change of androgen receptor ( AR) protein expression level on hypertensive vascular remodeling and the underlying mechanisms .Methods:Thirty rats were randomly allocated into 3 groups:control group, sham operation group and hypertensive group (10 rats in each group).The renovascular hypertensive rats model was established using the "2-kidney and 1-clip"method.Using tail-cuff method to measure the systolic blood pressure (pSB) and HE staining to observe the morphological structure of aorta , and the expressions of AR and MKP-1 were detected by immuno-histochemical technique .Results:For the morphological structure , there was no significant difference between the control group and the sham operation group , and also no significant difference in the expressions of AR and MKP-1 was found be-tween the two groups(P>0.05).Compared with the control group and the sham operation group ,the vascular remodeling of the aorta in the hypertensive group was remarkable , and the expressions of AR and MKP-1 significantly decreased ( P<0.001).In the hypertensive group, the expression of AR was positively correlated with the expression of MKP -1(r=0.657, P=0.039), and no such correlation was found in the control group or in the sham operation group (r=0.589,0.436,P>0.05).Conclusion:The vascular remodeling during hypertension is accompanied by the down-regulation of AR and MKP-1.%目的：探讨睾酮受体（ AR）蛋白表达水平的改变对高血压大鼠血管重构（ VR）的影响及其信号转导机制。方法：将30只大鼠随机分为对照组、假手术组、高血压组3组（每组10只）。采用“两肾一夹”法制备肾血管性高血压大鼠模型。 HE染色观察主动脉组织形态结构，免疫组化法检测3组大鼠主动脉组织中AR、丝裂原活化蛋白激酶磷酸酶-1（MKP-1）蛋白表达水平。结果：与对照组相比，假手术组主动脉形态结构基本正常，AR、MKP-1蛋白表达水平无显著改变（P＞0．05）；与对照组及假手术组相比，AR和MKP-1蛋白表达水平下降（P＜0．001）；且在高血压组AR与MKP-1蛋白的表达呈正相关（r＝0．657，P＝0．039），在对照组及假手术组无关（r＝0．589和0．436， P＞0．05）。结论：高血压VR过程中存在AR及MKP-1蛋白表达水平的改变。