氟病区成人雌激素受体α基因 PvuⅡ多态性与骨组织异常的关系

摘要

Aim:To explore the relationship between estrogen receptor α( ERα) gene polymorphism and bone tissue abnormalities in adults exposed to fluoride in drinking water .Methods:Adults aged from 18 to 55 years old who were born and grew or lived there for at least 5 years in endemic fluorosis villages or control villages were recruited using cluster sam-pling.They were allocated into endemic fluorosis group and control group in accordance with the above areas .PCR-RFLP was used to determine PvuⅡgenotype in ERαgene.X-ray was used to detect changes of the subjects′bone tissue of the two groups.Results:The abnormal rate of bone tissue in endemic fluorosis group (65.34%) were significantly higher than that in control group(40.32%)(P<0.05).The increased risk of bone tissue abnormalities was observed in subjects carry-ing Pp/PP genotype of ERαgene compared with subjects carrying pp genotype from endemic fluorosis group (PR=1.087, 95%CI=1.053-1.437;PR=1.145,95%CI=1.104-1.318).Conclusion: Bone tissue in people who carried ERαPvuⅡPp/PP genotype may be more susceptible to fluoride exposure .%目的：探讨雌激素受体（ ER）α基因PvuⅡ多态性与氟暴露地区成人骨组织异常的关系。方法：采用整群抽样方法，在氟病区及对照区选择当地出生或居住5 a以上18～55岁的人群为观察对象；采用PCR-RFLP法分析ERα基因PvuⅡ多态性；采用X线检查两区人群骨组织改变。结果：暴露组和对照组人群骨组织异常率分别为65．34％和40．32％，差异有统计学意义（P＜0．05）；以pp基因型为参照，暴露组携带Pp／PP人群骨组织异常的危险性增高（PR＝1．087，95％CI＝1．053～1．437或PR＝1．145，95％CI＝1．104～1．318）。结论：携带ERαPvuⅡPp／PP基因型可能增加骨组织对氟暴露敏感性。