基于 DNA 链置换反应的圆环形逻辑门设计

摘要

Using the DNA strand displacement technology that the reaction progress was programmable and the DNA strand dynamics was predictable,two logic computational models (logic NAND gate and NOR gate)with circular DNA as a basic work unit were designed based on DNA strand displacement reaction.In these mod-els,single-stranded DNA was regarded as the system input.Using many DNA recognition domains and toehold domains which were contained in circular DNA molecule,the output signal was accurately identified by detec-ting specific fluorescent signal to ensure the correctness and extensive applicability of these DNA logic gate models.%利用 DNA 链置换技术反应进程的可编程性和 DNA 链动力学特征的可预测性，基于 DNA 链置换反应，以圆环形 DNA 为基本单元，构造出与非门和或非门逻辑计算模型。该模型以单链 DNA 分子为输入信号，利用圆环形 DNA 分子包含的多个 DNA 识别区域和小支点区域，通过探测荧光信号精确识别其输出信号，来确保 DNA 分子逻辑门输出结果的正确性与广泛适用性。