BPA simulated wastewater was treated by Sequencing Batch Bioreactor( SBR) to investigate the effects of different BPA wastewater treatment methods on sludge acute toxicity and protein expression. The results showed that when the influent BPA concentration was 40 mg/L, the sludge activity in the BPAimpacted SBR was significantly inhibited, and the mass concentration of the effluent COD was significantly higher than that of the SBR gradually acclimated by the influent BPA. With the extension of running time, the mass concentration of BPA in the aqueous phase and mud phase increased first and then decreased until it was completely degraded. In the whole SBR operation, sludge and EPS acute toxicity in the SBR impacted by the BPA were both higher than that of the gradually acclimated SBR, but EPS acute toxicity had little effect on the acute toxicity of sludge; The protein expression in the sludge treated with different methods of BPA wastewater showed significant differences. The concentration of BPA and the way of degrading BPA had a significant effect on the protein expression in the sludge.%采用序批式生物反应器(SBR)处理BPA模拟废水,探讨不同BPA废水处理方式对污泥急性毒性和蛋白质表达的影响,结果表明:当进水BPA质量浓度为40 mg/L时,受BPA冲击的SBR中的污泥活性明显受到抑制,出水COD质量浓度显著高于经进水BPA逐步驯化的SBR,随着运行时间的延长,其水相、泥相中的BPA质量浓度均呈现先升高后降低直至被完全降解去除的变化趋势;在整个SBR运行过程中,经BPA冲击的SBR中污泥和EPS急性毒性均高于逐步驯化的SBR,但EPS急性毒性对污泥急性毒性的影响较小;采用不同方式处理BPA废水的污泥中其蛋白质表达呈现明显的差异,表明BPA质量浓度和降解去除BPA的方式对污泥中蛋白质表达有显著影响.
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