The tender leaves of Astilbe tabularis (Hemsl. ) Engler were used as explants, all suitable media of tissue culture and germplasm conservation in vitro were screened through uniform design experiments. The results showed that MS+6-BA1. 00 mg·L-1 + IBA0. 08 mg·L~' was most suitable for callus induction with a induction rate of 99. 7% ; MS+6-BA2. 80 mg·L-1 +IBA0. 02 mg·L-1 +GA30. 90 mg·L-1 was most suitable for shoots regeneration with a regeneration rate of 99. 5%; 1/6MS+IAA0. 02 mg·L-1 +IBA0. 04 mg·L-1 was most suitable for rooting with a rooting rate of 99. 2%; 1/4MS+6-BA0. 10 mg·L-1 +phloridzin 2. 50 mg·L-1 was most suitable for germplasm conservation in vitro for 27 months with a growing rate of 7. 9%. The results proved that tissue culture system of Astilbe tabularis (Hemsl. ) Engler has been successfully established, and the method of defering growth was utilized for germplasm conservation in vitro at normal temperature.%以山荷叶新生嫩叶为外植体,基于均匀设计法筛选其最适宜的离体培养和种质试管保存各阶段培养基.结果表明,最适合愈伤组织诱导的培养基为MS+6-BA 1.00 mg·L-1 +IBA 0.08 mg·L-1,诱导率为99.7％；愈伤组织再分化培养基为MS+6-BA 2.80 mg·L-1 +IBA 0.02 mg·L-1 +GA30.90 mg·L-1,分化率为99.5％；生根培养基为1/6MS+IAA 0.02 mg· L-1+IBA 0.04 mg·L-1,生根率达99.2％以上；试管保存培养基为1/4MS+6-BA0.10mg· L-1+根皮苷2.50mg· L-1,通过27个月的保存,芽苗生长率仅在7.9％以下.试验结果证明,成功建立了山荷叶离体培养体系,常温条件下利用延缓生长的方法在试管内保存山荷叶种质是可行的.
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