Objective: To screen the mi-RNA expression profile after interferon treatment in cells infected with hepatitis C virus (HCV). Methods: Huh-7. 5. 1 cells was infected with HCV by ire vitro transcription and cultured with interferon. The mi-RNA microarray was used to measure the mi-RNA expression in the control group, HCV transcription group and interference group. Intra-group differences were analyzed by the 2 ( -△△CT) method. Results: With mi-RNA expressed in normal Huh-7.5.1 cells as a benchmark, expressions of 13 kinds of mi-RN As were up-regulated after HCV infection and then down -regulated following interferon treatment; 7 were down-regulated after HCV infection and then up -regulated following interferon treatment. Conclusion: mi-RNA10a,mi-RNA21 ,mi-RNA149,mi-RNA152 and mi-RNA210 may be related to hepatitis C virus replication and transcription.%目的:探寻可能对丙型肝炎病毒(HCV)有作用的mi-RNA.方法:用体外转录得到的HCV感染正常的Huh-7.5.1细胞,然后加入干扰素作用,采用mi-RNA表达谱芯片技术,分别测定Huh-7.5.1细胞正常组和HCV感染组及干扰素作用组的mi-RNA的表达,采用2(- △△CT)法分析组间microRNA的表达情况.结果:以正常Huh-7.5.1细胞中表达的mi-RNA为基准,经过筛选发现13种mi-RNA在HCV感染后表达上调,而在干扰素治疗后则出现下调；另外还有7种mi-RNA则在HCV感染后出现表达下调,而在干扰素治疗后则发生上调.结论:mi-RNA10a、mi-RNA21、mi-RNA149和mi-RNA152及mi-RNA210可能与HCV的复制转录有关.
展开▼
