清肠栓防治大鼠溃疡性结肠炎作用的实验研究

摘要

Observe and study the morphological changes and effects on yoleperoxidase (MPO) of traditional Chinese medical complex Intestine-clearing Suppository (ICS) in preventing and treating ulcerative colitis (UC). Method.. Divide the experimental rats randomly into 6 groups, i. e., blank, salicylazosulfapyridine (SASP), model, high, middle and low dosage of ICS, except the blank group, makeother rats experimental UC model with albumen immunologic method of rabbit colon mucosa, further divide them randomly into 2 experimental groups, i. e. , prevention and treatment, afterwards at 2nd~21st day, give prevention group the medicine, 22nd~35th day, treatment group, then decapitate them and take the colon tissue, and observe it morphologically under optical microscope, measure active MPO, analyse statastic results. Result: Through general morphological observation ,we found there were serious congestion ,edema, mucosa erosion, many large ulcers in the rats of model group; less serious mucosa erosion and quantity of ulcers in SASP and low-dosage ICS groups: small ulcers, not serious mucosa erosion, low degree of congestion and edema in high-and middle-dosage ICS groups; the scores of inflammatory injury of ICS groups were markedly lower than that of model (P＜0. 01), meanwhile, the preventive and reating effectiveness of high-and middle-dosage groups was better than that of SASP (P＜0.05). Under the optical microscope, there were large area of mucosa coloboma in the rats of model group, the ulcer penetrated to muscular layer and serous coat; in SASP group and low-dosage ICS group, there were erosive mucosa and inflammatory cell infiltration, granulation on the base; in middle-and high-dosage groups, large part of colon mucosa recovered, granulation formed on the base, ulcers were obviously less than that in model group (P <＜0.01); there had marked difference compared with that in SASP group (P＜0.05). The MPO active values of high-and middle-dosage groups were markedly lower than that of model group (P ＜ 0.01). Conclusion: Traditional Chinese complex ICS had good prevention and treatment to rats UC, itseffectiveness was positively correlated with dosage and better than that of SASP suppository.%目的：观察研究中药复方清肠栓防治溃疡性结肠炎(UC)作用在形态学上的改变及其对髓过氧化物酶(MPO)的影响。方法：将实验大鼠随机分为空白组、模型组、柳氮磺胺吡啶(SASP)组、清肠栓高剂量组(清高组)、清肠栓中剂量组(清中组)、清肠栓低剂量组(清低组)6组，除空白组外，其余大鼠用兔结肠粘膜蛋白免疫法制作实验UC模型，并进一步随机分预防与治疗二大实验组，而后按预防组第2～21天，治疗组第22～35天分组给药后，断头取结肠组织，作大体形态学与光镜下观察，MPO活性测定，统计学结果分析。结果：大体形态学观察可见模型组大鼠严重充血、水肿、粘膜糜烂，溃疡多而且大；SASP组与清肠栓低剂量组粘膜糜烂程度减轻，有溃疡但数量少；清肠栓高、中剂量组溃疡小，粘膜糜烂不重，充血、水肿程度轻；清肠栓各组炎症损伤评分较模型组显著降低(P<0．01)，同时高、中剂量组防治效果优于SASP组(P<0．05)。光镜下观察可见模型组大鼠存在大范围粘膜缺损，溃疡深达肌层与浆膜层；SASP组与清低组粘膜糜烂，有炎细胞浸润，底部可见内芽；清肠栓中、高剂量组大部分结肠粘膜修复，底部有肉芽组织形成，溃疡生成情况较模型组显著减少(P<0．01)，和SASP组比较有显著性差异(P<0．05)。MPO活性值显示清肠栓高、中剂量组较模型组显著降低(P<0．01)。结论：中药复方清肠栓对大鼠UC具有良好的防治作用，其效果与用药剂量正相关，并优于SASP栓。