祛瘀化浊法对脂肪肝大鼠C反应蛋白和胰岛素抵抗的影响

摘要

[目的]观察祛瘀化浊法对酒精加高脂饮食诱导的脂肪肝大鼠血清胰岛素抵抗(IR)、C -反应蛋白(CRP)的影响,探讨其可能作用机制.[方法]将Wistar大鼠52只随机分为空白对照组、脂肪肝模型组、脂肪肝模型易复善治疗组、脂肪肝模型中药调脂积冲剂治疗组、脂肪肝模型中药(六味地黄丸)治疗组.采用酒精合并高脂乳剂建立大鼠脂肪肝模型,以易善复和六味地黄丸为阳性对照,8周后检测代、TG、HDL-C、LDL-C、ALT、GGT、FBG、血清胰岛素、CRP含量,评价胰岛B细胞功能,光镜观察组织学变化.[结果]模型组大鼠肝组织出现明显的脂肪变,其血清中TC、TG、LDL-C、ALT、GGT、FBG、FINS、HOMA-IR、CRP、肝指数均高于正常组,HDL-C低于正常组.调脂积冲剂能降低肝指数,降低CRP、TC、TG、ALT、GGT、TC、TG、LDL-C、FBG、FINS的含量,升高HDL-C的含量,降低HOMA-IR.[结论]CRP、IR参与酒精合并高脂饮食诱导的大鼠脂肪肝的发生,祛瘀化浊方药能在一定程度上能降低炎症状态,改善胰岛素抵抗,防止肝细胞脂肪变性和肝脏损伤的进一步发展,促进肝内脂质代谢.%[Objective] To observe the law on alcohol Quyu Huazhuo plus high-fat diet-induced fatty liver in serum insulin resistance (IR), C -reactive protein (CRP) the impact of its possible mechanism. [Methods] 52 Wistar rats, feeding adaptation in the laboratory after a week, were randomLy divided into control group, fatty liver model group, fatty liver model -good -treatment group, fatty liver model of traditional Chinese medicine treatment group Tiaozhi plot granule , Fatty liver model of traditional Chinese medicine (Liuweidihuang pill) in the treatment group.Use of alcohol in the establishment of a joint high fat emulsion of fatty liver model to Essentials and Liuweidihuang pill for the positive control, the use of automatic biochemical analyzer detected TC, TG, HDL-C, LDL-C, ALT, GGT, FBG, using ELISA serum insulin levels.B islet cell function evaluation method used to assess steady-state mode (HOMA). Double-antibody sandwich enzyme immunoassay determination of CRP levels. By light microscopy Watch changes.[Results] The model of rat liver tissue significantly change the fat, serum TC, TG, LDL-C, ALT, GGT, FBG, FINS, HOMA-IR, CRP, liver index higher than the normal group, HDL-C Lower than normal group. Tiaozhi plot granules can reduce the liver index, lower CRP, TC, TG, ALT, GGT, TC, TG, LDL-C, FBG, FINS content, increased HDL-C levels lower HOMA-IR. [Conclusion] CRP, IR involved in the merger of alcohol-induced high-fat diet of fatty liver in rats, Quyu Huazhuo Prescriptions can significantly lower serum CRP levels, improve the IR, control the development of fatty liver.