绞股蓝和高脂血症对雄性兔抗精子抗体的影响研究

摘要

[Objective]Observe the influence of the different raising condition to the adult Male rabbit antispermatozoon immunebody (AsAb).The discussion influenced mechanism of Fiveleaf Gynostemma Herb to AsAb of the adult male rabbit.[Methods]40 male rabbits were divided into group A, group C, group D, group E at random.Each was fed fatty diets added Fiveleaf Gynostemma Herb 5g/kg, fatty diets, standard diets added Fiveleaf Gynostemma Herb and standard diets.Blood was extracted seperately before the experiment and the end of the third, sixth and the nineth week after the experiment to check the level of TG, TC, LDL-C, HDL-C.The experiment ended, AsAb of rabbit was examined through qualitative and quota.The microstructures of the testicle were also observed by a microscope.[Results]① TG,TC, LDL-C and HDL-C: With the feeding time prolonged, TG, TC,LDL-C of group A and group C were growing gradually, HDL-C of group A was growing and had the difference.Group A was lower than groupc.after the feeding,TG,TC, LDL-C of group A were lower than to group C, HDL of group A was superior to group B.③ AsAb inspection:in qualitative tests,C group of 4 cases AsAb masculine gender, A group of 1 case masculine gender, D and E group of AsAb is negative.In quantitative experiment,AsAb of group A was growing gradually.AsAb of group C was growing gradually.After the feeding, AsAb of group A was lower than to group C.Microstructures of the testicle: group A, group D,group E of freshsperm cell was approximately normal; C group of testicles fresh spermcells was fat denatured, the basis membrane accumulations and the spermatide quantity drop.[Conclusion]The high lipemia caused the male rabbit AsAb masculine gender.Fiveleaf Gynostemma Herb throughed to reduce the blood fats, improved blood fats metabolism AsAb which caused the high lipemia had the inhibitory action.Fiveleaf Gynostemma Herb can't cause the standard feed raising male rabbit AsAb masculine gender.%[目的]观察不同饲养条件对成年雄性兔抗精子抗体(AsAb)的影响,探讨绞股蓝对雄性兔AsAb的影响及机理.[方法]40只成年日本雄性大耳白兔随机分为A组、C组、D组、E组,分别喂饲高脂饲料+绞股蓝5g/kg、高脂饲料、标准饲料+绞股篮5g/kg、标准饲料.实验前和实验开始后3周、6周、9周末检测甘油三脂(TG)、胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C).第九周处死动物,定性和定量检测兔AsAb并观察睾丸显微结构.[结果]①TG、TC、LDL-C、HDL-C:A组、C组随饲养时间延长,TG、TC、LDL-C渐升,与饲养前比较有显著差异(P＜0.05～0.01),A组HDL-C渐升,与饲养前比较有显著性差异(P＜0.01);饲养后A组TG、TC、LDL-C低于C组,A组HDL-C高于C组,饲养后组间比较有显著差异(P＜0.05～0.01).②AsAb检查:在定性实验中,C组4例AsAb阳性,A组1例阳性,D组、E组AsAb均为阴性.在定量实验中,饲养前后A组组内比较有显著差异(P＜0.05),C组组内比较有非常显著差异(P＜0.01),饲养后A组与C组比较有非常显著差异(P＜0.01).③光镜检查:A组、D组、E组生精细胞正常;C组睾丸生精细胞脂肪变性,间质纤维化,基底膜增厚,精子细胞数量下降.[结论]高脂血症导致雄性兔AsAb阳性,绞股蓝可能通过降低血脂、改善血脂代谢对高脂血症导致的AsAb具有抑制作用.绞股篮不会导致标准饲料饲养雄性兔AsAb阳性.