HPLC法检测冬凌草甲素壳聚糖微球包封率及载药量

摘要

[Objective]To establish HPLC method to determine drug content and entrapment efficiency of oridonin chitosan microspheres. [Method] Oridonin chitosan microspheres were broken by acid reflux,drug content and entrapment efficiency of the microsphere were detected by HPLC. The chromatographic conditions:methanol:water (50:50) as mobile phase, the separation was performed on a Kromasil C18 column(50 mm ×4.6 mm , 5μm) at a flow rate of 1.0 mL/min. The detection wavelength was 238 nm and the column temperature was 30 ℃. [Results]The drug content of oridonin chitosan microphere was 2.238%, the encapsulated efficency was 0.789%.[Conclusion]There is good stability detection of oridonin in chromatographic condition.%[目的]建立冬凌草甲素壳聚糖微球载药量和包封率测定的高效液相色谱分析方法。[方法]采用酸回流破乳法对制得的冬凌草甲素壳聚糖微球进行破乳，采用高效液相色谱法测定微球的载药量和包封率。色谱条件：流动相：甲醇-水（50：50）；Kromasil C18柱（50mm ×4.6mm，5μm）；流速：1.0 mL/min；检测波长：238 nm；柱温：30℃。[结果]当冬凌草甲素投药量与载体的质量比为4：10时，载药量为2.238%，包封率为0.789%，达到最高值。[结论]此色谱条件对冬凌草甲素的检测具有良好的稳定性。