活络效灵丹通过抑制脑组织炎症反应对缺血性脑损伤的保护作用

摘要

[目的]观察活络效灵丹是否能抑制脑缺血后炎症反应。[方法]使用朗格（Longer）线栓塞法大鼠脑缺血再灌注损伤模型，90只成年雄性SD大鼠，体重200～250g。随机分为5组： A、B、C、D、E组，每组6只大鼠。A为假手术组(sham)；B为损伤对照组（给予与药物等体积的生理盐水）；C、D，E组分别给与活络效灵丹（HLXLD ）0.5、2.0、6.0 g·kg-1。采用TTC染色脑梗塞范围测定观察活络效灵丹对大鼠脑缺血损伤的作用，采用实时定量反转录PCR观察活络效灵丹对脑组织炎症反应介质肿瘤坏死因子（Tumor Necrosis Factor-alpha，TNF-α），白介素1（Interleukin-1 beta，IL-1β）的影响,采用蛋白质免疫印迹实验（Western Blot）及磷酸化p-65蛋白抗体观察p-65蛋白磷酸化水平改变。[结果]与生理盐水对照组比较，活络效灵丹可以明显抑制缺血再灌注大鼠脑组织炎性因子的表达水平，显著缩小大鼠脑缺血再灌注损伤脑梗塞范围，明显改善脑缺血神经功能缺失症状。活络效灵丹明显抑制脑组织炎症信号通路NF-κB磷酸化反应。[结论]活络效灵丹对大鼠脑缺血再灌注损伤起保护作用可能与其抑制脑组织炎症信号通路NF-κB磷酸化反应有关。%[Objective]To observe whether Huo-Luo-Xiao-Ling-Dan(HLXLD) can inhibit the inflammation after ischemic stroke. [Methods] Focal cere-bral ischemia was induced by middle cerebral artery occlusion (MCAO) for 2 hours fol owed by 24 or 48 hours reperfusion in male rats. We treated is-chemic stroke rats with HLXLD(0.5, 2.0 and 6.0g·kg-1) by daily gavages beginning at the onset of stroke. The control group received the vehicle. [Re-sults] HLXLD 2.0, 6.0/kg treatment significantly attenuated the infarct volume, neurological deficits, upregulation of proinflammatory cytokines in the brain of rats after stroke compared with that of non-HLXLD group. Furthermore, the activation of NF-κB signaling was significantly reduced in HLXLD treated rats after stroke compared to that of non-HLXLD group. [Conclusion] Our data demonstrated that HLXLD can play an important protective role in brain injury after ischemic stroke via inhibiting inflammatory responses in the brain.