原代培养的新生大鼠小脑颗粒神经元经5mmol/L KCl或25mmol/L KCl或5mmol/L KCl+10μmol/L FK处理，用二乙酸荧光素(FDA)染色法测定存活细胞数及用碘标放射免疫法测定神经细胞内cAMP浓度(［cAMP］i)。结果表明：①幸存神经元数：高钾组199.11 ±24.00，低钾组47.56±11.09，FK组201.11±27.43；②［cAMP］i(pmol/孔)：高钾组0.58±0.220，低钾组0.32±0.106， FK组2.20±0.469。认为高K+阻止原代培养新生大鼠小脑颗粒神经元凋亡的效应与cAMP无关。%The cerebellar granular neurons in newborn primary cultured rats were treated with 5 mmol/L KCl or 25 mmol/L KCl or 5 mmol/L KCl plus 10 μmol/L FK. The neuron survival was detected by fluorescein diacetate (FDA) staining. The intracellular cyclic AMP ［cAMP］i was quantitated by using 125I radioimmunoassay. Results showed that the neuronal survival was 199.11±24.00 in the high K+ group, 47.56±11.09 in the low K+ group and 201.11±27.43 in the FK group. Additionally, the ［cAMP］i (pmol/well) was 0.58±0.220 in the high K+ group, 0.32±0.106 in the low K+ group and 2.20±0.469 in the FK group. This data indicates that the prevention of apoptosis of primary cultured rat cerebellar granular neurons by high K+ had nothing to to with cAMP.
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