乙型肝炎病毒感染对精子DNA完整性影响的研究

摘要

Objective To study the effects of hepatitis B virus infection on sperm DNA integrity. Meth-ods Twelve male patients with seropositive hepatitis B surface antigen (HBsAg)were selected into the obser-vation group,ten male individuals undergone healthy check-up were chosen into the control group.A sperm chromatin dispersion (SCD)test and a semen routine detection were performed for examining semen samples of the two groups. Results compared control group with observation group,the observation group had obvi-ously lower sperm density,lower ratio of forward motile sperm,lower motility rate and lower normal mor-phology rate,there were statistical significant differences (P<0.05).Comparison of semen volume between the two groups showed that there was no significant difference (P >0.05).Comparing the sperm fragment SCD test results between control group and observation group showed there were statistical differences (P <0.05). Conclusion Hepatitis B virus infection affects various sperm indexes such as sperm motility and sperm density,which seriously impacts on the quality of sperm DNA and results in a great decline in sperm quality.%目的：探讨乙型肝炎病毒感染对精子DNA完整性的影响。方法选择血清乙型肝炎病毒表面抗原检测为阳性的12例男性患者作为观察组，对照组选择健康体检男性10例，对两组精液样本进行精子染色质扩散(SCD)实验和精液常规检测。结果与对照组相比，观察组精液密度、前向活动率、活动率和正常形态率明显更低，差异具有统计学意义(P<0.05)；在精液量的对比上，两组差异无统计学意义(P >0.05)。与对照组对比，观察组精子碎片 SCD 检查结果中，各项数据对比差异具有统计学意义(P<0.05)。结论乙型肝炎病毒感染影响精子的活动能力和精子密度多方面指标，严重影响精子DNA的质量，导致精子的质量大幅度下降。