ITO电极上催化伏安法测定锇联吡啶的研究

摘要

An electron transfer mediator osmium bipyridine was synthesized. The electrochemical behaviors of osmi- um bipyridine at ITO electrode were studied and the catalytic reaction kinetics of it in system of glucose/glucose ox- idase was measured. The homogeneous reaction rate constant (Ks ) is 2.07 ×10^-2cm/s, and the first- order reac- tion rate constant (Kf) and second -order reaction rate constant (Kmed) in catalyzed reaction are 1.05 × 10^-3s-1 and 1.57× 10^4 L/( mol. s), respectively. The detection limited is 0. 016 μmol/L. Therefore, as an electrochemical label, osmium bipyridine can be used to construct biosensor for the catalyzed signal amplification.%合成了电子转移介体锇联吡啶Os（bpy）3，采用催化伏安法研究了Os（bpy）3在ITO电极上的电化学行为，并对Os（bpy）3在葡萄糖一葡萄糖氧化酶体系中的催化反应动力学常数进行了测定。Os（bpy）3的均相反应速率常数Ks为2．07×10^-2cm／s，催化反应中一级反应速率常Kf为1．05×10^-3s^-1，二级反应速率常数kmed为1．57×10^4L／（mol．s）。并采用催化伏安法测定Os（bpy）3的检出限为为0．016μmol／L。因此，Os（bpy）3作为电化学标记物，能够用于生物传感器的构建。