快速心房起搏制作猪心动过速心肌病动物模型研究

摘要

Objective To evaluate the feasibility of developing porcine model of tachycardia - induced cardiomyopathy by rapid atrial pacing. Methods Eight healthy piglets underwent continuous right atrial pacing at 400 beats/min for 2 weeks while two healthy piglets served as normal control. Left ventricular end - systolic volume ( LVESV) , left ventricular end - distolic volume (LVEDV) and left ventricular ejection fraction ( LVEF) were measured by echocardiography . The atrial natriuretic peptide (ANP) concentration was detected before and after rapid atrial pacing . The left ventricular sections were analyzed histologically through hematoxylin and eosin ( H - E) staining. Results Compared to baseline , the LVESV , LVEDV and the level of ANP all increased significantly after 2 weeks rapid pacing (P < 0. 05) , while LVEF decreased significantly [ (61. 6±7. 2)% vs (38.5±5. l)%,P<0. 05]. Left ventricular tissues demonstrated ventricular tissue disorder, focal necrosis , muscle dissolution , edema, interstitial collagen connective tissue proliferation and in -flammatory cell infiltration. Conclusion Short term rapid atrial pacing is an convenient and effective method for develo -ping porcine model of tachycardia - induced cardiomyopathy.%目的 探讨通过快速心房起搏构建猪心动过速性心肌病模型的可行性.方法 10只健康小猪经穿刺颈静脉途径植入AAI型起搏器,8只给予400次/min的快速右心房起搏2周,2只不起搏作为对照组,起搏前后应用超声心动图观测收缩、舒张末期左心室容积大小及左心室射血分数(LVEF),并检测血心房钠尿肽(ANP)水平;通过苏木精-伊红染色观察左心室心肌病理改变.结果 快速起搏2周后,猪左心室收缩末期容积(LVESV)和舒张末期容积(LVEDV)均显著增加(P<0.05);血浆中ANP显著增加,苏木精-伊红染色可见心室组织细胞排列紊乱,出现局灶性坏死、肌溶解、水肿、间质胶原结缔组织增生、炎性细胞浸润.结论 短期快速心房起搏是建立猪心动过速心肌病动物模型简便而有效的方法.