Objective To investigate the protective effects of curcumin pretreatment on the gastric ischemia/reperfu-sion (GI/R) injury in rats.Methods A rat model of GI/R injury was established through occlusion of the celiac artery in rats for 30 min followed by reperfusion for 60 min.Then the area of damaged gastric mucosa was calculated by soft-ware, while the index of gastric mucosal damages was assessed according to the white grading method.The amounts of Bcl -2 and Bax in the tissues of gastric mucosa were detected using immunohistochemical methods and image analysis. Results Compared with the control, rats in the GI/R group presented damages over remarkably larger areas and higher damage indices (P <0.05 or P <0.01).Curcumin pretreatment could relieve the gastric mucosa injury caused by GI/R. The area of gastric mucosal damages and the damage index became significantly lowered with the rising of the concentra-tions of curcumin (P <0.05 or P <0.01).Meanwhile, curcumin pretreatment could weaken the amounts of Bax but en-hance the level of Bcl -2 in the gastric mucosal tissues after GI/R in a dose -dependent manner (P <0.05 or P <0.01).Conclusion Curcumin pretreatment can protect the gastric mucosa and attenuate its damages caused by GI/R through enhancing Bcl -2 but weakening Bax protein expression.This finding indicates the potential role of curcumin as a protective agent against organ injuries caused by ischemia/reperfusion.%目的：观察姜黄素预处理对大鼠胃缺血／再灌注（gastric ischemia －reperfusion，GI／R）损伤的保护作用。方法采用夹闭大鼠腹腔动脉30 min 再灌注1 h 建立 GI／R 损伤模型。应用计算机软件分析计算 GI／R 引起的胃黏膜损伤面积，根据 whtie 评分法计算胃黏膜损伤指数，用免疫组化法与图像分析技术检测胃黏膜组织 B 细胞淋巴瘤因子2（Bcl －2）与 Bcl －2相关 X 蛋白（Bax）表达量。结果与对照组相比，GI／R 组胃黏膜损伤面积和损伤指数明显增加（P ＜0．05，P ＜0．01）；姜黄素预处理能够有效减轻 GI／R 引起的胃黏膜损伤，随着姜黄素浓度的增加，GI／R 引起的胃黏膜损伤面积和损伤指数显著减少，并具有浓度依赖性（P ＜0．05，P ＜0．01）。同时，姜黄素预处理能够降低 GI／R 引起的胃黏膜组织 Bax 蛋白表达，增加胃黏膜组织 Bcl －2蛋白表达，且随着姜黄素浓度的增加，其差异具有浓度依赖性（P ＜0．05，P ＜0．01）。结论姜黄素预处理通过增加抗凋亡蛋白 Bcl －2、减少促凋亡蛋白 Bax 的表达有效抑制 GI／R 引起的胃黏膜损伤，对胃黏膜起到一定程度的保护作用，对临床缺血／再灌注引起的器官损伤具有潜在的治疗作用。
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