慢性阻塞性肺疾病患者单核细胞NF-κB活性的研究

摘要

目的 探讨单核细胞NF-κB的激活在COPD发病机制中的作用.方法 抽取31例急性加重期组和26例稳定期组的COPD患者外周血,分离单核细胞并培养,免疫细胞化学法测定核转录因子NF-κB p65的活性表达;同时检测外周血SOD、MDA、IL-8浓度,所有患者均测定肺功能(FEV1占预计值% 、FEV1/FVC).并以15例健康志愿者作为对照组.结果 LPS刺激后,急性加重期组单核细胞NF-κB p65表达显著高于稳定期组及正常组(P＜0.01),稳定期组高于正常组(P＜0.01).经LPS刺激后,各组单个核细胞NF-κB p65活性均显著增高,(P＜0.05);外周血单核细胞NF-κB的激活与血清IL-8、MDA水平呈正相关,而与SOD呈负相关.结论 NF-κB在COPD单核细胞表达细胞因子IL-8的信号转导调控中起重要作用.%Objective To investigate the role of NF-κB activation in the inflammatory mechanism of chronic obstructive pulmonary disease (COPD).Methods Monocyte were collected from patients with COPD and were cultured,and stimulated with lipopolysaccharide (LPS) ; NF-κB p65 activation was measured by immunohistochemistry; SOD ,MDA and IL-8 and lung function were determined synchronously.Results The NF-κB p65 was induced by LPS in monocyte in all subjects, but it was most markedly done in COPD patients with exacerbateions; There was positive correlation between the NF-κB p65 activation of monocytes and levels of IL-8 and MDA in circulation, but it was negative correlation to SOD.Conclusion NF-κB plays a vital role in regulating product of IL-8 in monocyte in COPD.