大鼠下颌前导后髁突软骨组织中 BMP-2的含量变化研究

摘要

Objective To observe the changes of Bone Morphogenetic Protein-2 (BMP-2)in the cartilage of rat condyle after mandibular constantly advancement and its impact on the condylar cartilage.Methods A total of 64 male rats were randomly divided into experimental and control groups.In the experimental group,each rat were worn on the bite-jumping appliances in order to advance the mandible for 24 hours. The rats were executed in each group after 0 d,1 d,3 d,5 d,1 w,2 w,4 w and 8 w.BMP-2 content was detected by enzyme linked immunosorbent assay (ELISA).Results (1)The BMP-2 content was increased in the condylar cartilage.Compared with the control group,the difference was statistically significant (P=0.000).Along with the strength time progress,BMP-2 content also changed,the BMP-2 content of each time point was different significantly (P =0.011).(2)After wearing of bite-jumping appliances,BMP-2 content in the mandibular condylar chondrocytes and time present a quadratic trend (parabolic)model,and reflect a certain amount of time rhythm (P =0.000).Conclusion The bite-jumping appliance can promote the release of the condylar chondrocytes BMP-2 levels,which could participate in the process of condylar cartilage bone reconstruction.%目的：探讨下颌前导对大鼠髁突软骨细胞骨形成蛋白-2（BMP-2）含量的影响。方法64只雄性 SD大鼠随机分为实验组和对照组，实验组大鼠全天佩戴自制的前导装置，对照组不佩戴矫治器。于第0、1、3、5天及第1、2、4、8周分别处死各组大鼠，应用酶联免疫吸附法（ELISA）定量检测、分析大鼠髁突软骨内 BMP-2的含量变化。结果（1）与对照组比较，实验组大鼠髁突软骨 BMP-2含量增高，差异有统计学意义（P ＝0．000）。随加力时间进展，BMP-2含量也随之改变，各时间点 BMP-2含量比较差异有统计学意义（P ＝0．011）。（2）下颌前导加载后髁突软骨细胞 BMP-2含量与时间呈二次项（抛物线）模型趋势，体现一定的时间节律（P ＝0．000）。结论下颌前导能够促进髁突软骨细胞 BMP-2的释放含量，从而可能参与髁突软骨的骨改建过程。