TRβ1蛋白在人类乳腺癌组织中的表达及临床意义

摘要

Objective To investigate the expression and clinical significance of thyroid hormone receptorβ1 (TRβ1)in breast cancer tissues by detecting the expression of TRβ1 in human breast cancer tissues and adj acent tissues.Methods 112 cases of breast cancer and adj acent tissues were collected from the Fifth Affiliated Hospital of Xinjiang Medical University and Affiliated Tumor hospital of Xinjiang Medical University,which were confirmed by clinical pathology.Immune-histochemical streptavidin biotin peroxidase (SP) was used to detect the expression of TRβ1 in breast cancer tissue and adj acent tissues.We investigated the clinical data of these 112 cases of breast cancer patients.The relationship of expression TRβ1 and clinical pathological data were analyzed.Results The positive expression of TRβ1 was mainly localized in the nucleus and cytoplasm.The positive expression of TRβ1 in human breast cancer tissues was significantly lower than that in adjacent normal tissues.The positive expression rate of TRβ1 in breast cancer tissue was 47. 32%(53/112),significantly lower than that in adjacent normal tissues (100%,112/112).The low expression of TRβ1 in breast cancer was closely related with lymph node metastasis (χ2=14.837,P <0.001), histological grade (χ2=8.459,P =0.015),Her-2 state (χ2=4.579,P =0.032)and clinical stage (χ2=4. 177,P =0.041),while which was not associated with age,nationality,primary tumor size,ER,PR, genotype (P >0.05).Conclusion Compared with the adjacent tissues,the positive expression of TRβ1 was lower in breast cancer than that in adj acent tissues.In breast cancer tissue,the higher of histological grade,the lower of positive expression rate of TRβ1 .The negative expression of TRβ1 are related to late clinical stage,lymph node metastasis and other adverse prognostic factors.The positive expression of TRβ1 are not associated with the molecular classification and prognosis of breast cancer.%目的 探讨TRβ1在人类乳腺癌组织及癌旁组织的表达情况及其在乳腺癌组织中表达的临床意义.方法 收集112例新疆医科大学第五附属医院和附属肿瘤医院经临床证实的乳腺癌患者的乳腺癌组织及癌旁组织石蜡标本,使用免疫组织化学链霉素抗生物素-过氧化物酶(SP)法检测112例乳腺癌组织及癌旁组织中TRβ1蛋白的表达,并调取112例乳腺癌患者临床资料,分析乳腺癌组织中TRβ1蛋白表达与临床病理资料之间的关系.结果 TRβ1阳性表达主要定位于细胞核和细胞质.TRβ1在人类乳腺癌组织中阳性表达显著低于癌旁正常组织,乳腺癌组织中TRβ1蛋白阳性表达率为47.32%(53/112),显著低于癌旁正常组织(100%,112/112);乳腺癌组织中TRβ1蛋白异常低表达与淋巴结转移(χ2=14.837,P<0.001)、组织学分级(χ2=8.459,P=0.015)及Her-2状态(χ2=4.579,P=0.032)、临床分期(χ2=4.177,P=0.041)密切相关,与年龄、民族、原发肿瘤大小、ER、PR、基因分型无关(P>0.05).结论 相比癌旁组织,乳腺癌组织中TRβ1阳性表达低;乳腺癌组织学分级高者TRβ1阳性表达率低;TRβ1阴性表达与临床分期晚、淋巴结转移等乳腺癌不良预后因素相关;在乳腺癌组织TRβ1阳性表达与乳腺癌分子分型及预后无关.