The silk fibroin/gelatin porous scaffold was prepared by the freeze-drying method. The temperature was set as-20℃ and -70℃, respectively. The micro-porous morphology was observed by SEM and the porosities were measured. The swelling performance and mechanical property were also investigated. HepG2 cells were cultured in the two kinds of scaffolds to evaluate their biocompatibility. The results show that with decrease of the freezing temperature, the pore size becomes smaller and the porous morphology gets more homogeneous. Reducing freezing temperature results in higher porosity and much stronger mechanical performance. The cell culture results demonstrate that with the decrease of freezing temperature, fewer cells adhere to the scaffold. Cells in scaffolds with higher freezing temperature proliferate gradually. Cells in scaffolds at lower freezing temperature proliferate very well at first, while the proliferation is limited later. It is concluded that the silk fibroin/gelatin porous scaffold can be successfully prepared by the freeze-drying method, and the porous structure and properties of the scaffold can be controlled by adjusting the freezing temperature.%采用冷冻干燥法制备了蚕丝蛋白/明胶多孔支架,并分别在-20℃和-70℃预冻,以观察支架的微孔结构,测量支架的孔隙率、溶胀吸水性及力学性能,研究HepG2细胞在支架中的生长增殖情况.结果表明:随预冻温度降低,支架的孔径减小且微孔分布更加均匀,孔隙率及力学性能均得到提高.细胞培养结果显示:随预冻温度降低,支架的细胞贴壁率减小,-20℃预冻支架中的细胞数量稳步增加,而-70℃预冻支架中的细胞在培养前期生长良好,在培养后期增殖受到限制.此项研究证明,采用冷冻干燥法可以成功制备蚕丝蛋白/明胶多孔支架,通过改变预冻温度可以控制支架的孔隙结构及性能.
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