针灸对IL2-IFN-NKC调节网与其相关网络MΦ-IL1-Th的效应—针灸并施对荷瘤(S37)小鼠相关网络的效应

摘要

Objective The tumor-bearing (S37) mice were used to study theeffects of acupuncture and moxibustion on relative network of IL2-IFN-NKC regulatory network.Methods After puncturing and (or) igniting the acupoints of Dazhui(Du 14) and Houhai(Du 1), we observed its effects on MΦ,IL1,Th and tumor S37 by means of MΦ devouring neutral red test,MTT colorimetry and Anti-L3T4 McAb-SPA rosetle test simultaneously.Results The phagocytic power of MΦ in the mices abdomen,the amount of IL1 and percentage of L3T4 increased obviously,compared to that of the control group;excepted the acupuncture group,the other two experimental groups had the obvious inhibition to the tumor S37. In addition,the IL1 content increased remarkably in acumoxi group and moxibustion group than in acupuncture group. The percentage of L3T4 increased remarkably in acumoxi group than that in acupuncture group.Conclusion Applying both acupuncture and moxibustion at points of Dazhui(Du 14) and Houhai(Du 1) can make positive regulation on the relative MΦ-IL1-Th network of IL2-IFN-NKC regulatory network and has anti-tumor effect.The moxibustion also has the similar effects.%目的 以荷瘤(S37)小鼠为模型，进一步验证针灸并施的抗肿瘤和对IL2-IFN-NKC调节网的相关网络MΦ-IL1-Th的效应。方法 针灸荷瘤(S37)小鼠的大椎、后海二穴后，取样，以吞噬中性红比色分析法，MTT比色分析法和单抗SPA-Ig花环法等观察针灸对MΦ、IL1、Th及S37肉瘤的影响。结果 荷瘤针灸并施组、荷瘤艾灸组、荷瘤针刺组的小鼠腹腔MΦ吞噬功能、IL1含量及Th细胞(L3T4)百分率均较荷瘤对照组显著增高；除针刺组外，其余两实验组均对S37肉瘤有明显抑制作用。另外，针灸组和艾灸组IL1含量均较针刺组显著增高；针灸组Th细胞(L3T4）较针刺组显著增高。结论 针灸荷瘤小鼠大椎、后海二穴，具有正向调节IL2-IFN-NKC网的相关网络MΦ-IL1-Th和抑瘤(S37)作用。艾灸也显示出相似的效果。