Objective To explore changes of estrous cycle and ovarian morphology in amygdale-kindled seizures female rats. Methods Thirty-five SD female rats aged 8 weeks were randomly divided into epilepsy model (n=15), sham-operation (n = 10) and control groups (n=10). Kindling electrodes were implanted in the left basolateral amygdala in the female rats of model group. The rats were kindled by brief, daily, suprathreshold stimulations with a bipolar electrode. The sham-kindled rats only had bipolar electrode implanted in the left basolateral amygdala. Controls were normal female rats. Estrous cycles were monitored with daily vaginal smears. Electrographic and behavioral seizures were monitored, right ovary tissues were taken after 20 days of kindled in the diestrus. At the same time, sham-kindled subjects and their yoked controls were sacrificed and their right ovaries were taken in their diestrus. Ovarian morphometric analyses were made. Results Kindled rats lost ovarian periodicity and they displayed significant weight gain and ovarian increase (P＜0.05). The results of HE staining showed that compared with control group and sham-kindled group, kindled female rats had enlarged ovaries with numerous growing follicles, especially many preantral follicles, and the total number of follicles and atretic follicles increased (P＜0.05). The ovarian in model group exhibited polycystic changes. Conclusion Seizures initiated in amygdala result in polycystic ovary and loss of ovarian periodicity.%目的 探讨大鼠动情周期及卵巢形态学在杏仁核电刺激点燃的癫痫模型中的变化.方法 35只8周龄SD雌性大鼠,随机分为癫痫模型组(n=15)、假手术组(n=10)和对照组(n=10).癫痫模型组,双极电极植入大鼠左侧杏仁基底外侧核,给予连续电刺激,建立电点燃的癫痫模型;假手术组,电极植入后不给予电刺激;对照组为正常雌性大鼠.所有大鼠每天清晨行阴道细胞涂片,以监测动情周期.癫痫模型大鼠在点燃20d后于动情周期的间期取右侧卵巢组织,同期其他两组大鼠也分别于动情间期摘取右侧卵巢组织,制作病理切片,HE染色后镜下阅片,采用Image-Pro Plus 6.0分析软件进行形态学分析.结果 癫痫模型大鼠失去正常动情周期,日均体重获得及卵巢重量均较假手术组及对照组显著增高(P<0.05);病理显示,癫痫模型组大鼠卵巢中窦前卵泡、总卵泡数及闭锁卵泡显著增多(P<0.05),卵巢发生多囊性改变.结论 癫痫发作可影响大鼠排卵功能,并导致大鼠发生卵巢多囊性改变.
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