目的 研究在过氧化物酶体增长因子活化受体δ(PPARδ)表达中的相关调控因子.方法 用RT-PCR扩增人类PPARδ启动子序列,通过Deletion及观测各重组体转染活性,通过电泳迁移率变更分析(EMSA)、突变等方法确定核转录因子激活蛋白1(APl)及NF-κB对PPARδ表达的作用.结果 Deletion及重组转染后发现AP1及NF-κB因子各自结合位点突变后转染活性明显降低,同时突变其转染活性无明显变化.结论 AP1及NF-κB均可以增强PPARδ的表达活性.%Objective To study the regulatory effects of activator protein 1 (AP1) and NF-KB on the PPARδ gene in Lovo cells. Methods By using methods of deletion, transient transfection, Gel-Shift and mutation, the regulatory effects of AP1 and NF-ΚB on PPARδ gene in Lovo cells were observed. Results Transient transfection results showed that the regions from 3 361 to 3 464 were sufficient to confer increased expression in Lovo cells. And there was the region involved in protein binding as shown in Gel-Shift assays. Mutation suggested that the AP1 and NF-ΚB within the 5'-UTR of PPARδ constituted a binding site for a potential transcriptional activator in Lovo cells. Conclusion Our data indicate that AP1 and NF-ΚB are potential transcriptional enhancers for PPARδ.
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