Highly Sensitive Spectrofluorimetric Determination of Riboflavin Based on the Generation of Active Oxygen Coupled with Enzymatic Reaction

摘要

A spectrofluorimetric method for the determining riboflavin (VB 2 ) based on its enhancement on the fluorescence of hemoglobin|catalyzed enzymatic reaction was proposed. The proposed method consisted of two reactions. One was the photochemical reaction of VB 2 , the other was a hemoglobin|catalyzed enzymatic reaction. The optimal experimental conditions for the determinations were established. The linear range of the method was 5.0×10 -9 |1.0×10 -7 mol/L of VB 2 . The detection limit was calculated to be 3.65×10 -9 mol/L. The relative standard deviation of this method was 2.3 % at 7.0×10 -8 mol/L for 11 determinations.