自由人槭‘秋焰’休眠芽的离体培养∗

摘要

To establish rapid-propagation system of in vitro culture of Acer × freemanii‘Autumn Blaze ’ , dormant buds of Acer×freemanii‘Autumn Blaze’ was used as explants, and different sterilization method and different medi-um were used to conduct in vitro process and multipliction culture study. The results showed that the survival rate reached over 80% and the inoculation effect was the best when the stem explants were sterilized by 0. 1% HgCl2 with 80-120s, and then the bud scales was stripped to expose leaf primordium. The optimal induction medium was Improved MS+0. 5mg/L 6-BA+0. 02mg/L IBA+0. 05mg/L ZT, and the germination rate reached to 64. 44%. The optimal proliferation medium was Improved MS+0. 02 mg/L IBA+0. 01 mg/L ZT, and the multiplication coef-ficient achieved to 6. 5 .%为建立自由人槭‘秋焰’休眠芽的离体快繁体系,以其休眠芽(保留芽苞鳞片与剥去芽苞鳞片)为外植体,采用不同消毒方法和不同培养基进行离体启动和增殖培养研究。结果表明,带休眠芽茎段外植体用0．1%HgCl2消毒80~120s后,将芽苞鳞片剥去露出叶原基接种效果最佳,成活率可达80%以上。其休眠芽最适萌发培养基为改良MS+0．5mg/L 6-BA+0．02mg/L IBA+0．05mg/L ZT,萌发率可达64．44%；最适增殖培养基为改良MS+0．02mg/L IBA+0．01mg/L ZT,增殖系数达6．5。