为探究利用脲酶活性细菌调控土壤脲酶活性的可行性,以土壤、 土壤关联脲酶活性材料及土壤无关联脲酶活性材料为对象,采用土壤理化性质分析、 脲酶活性细菌分离及其系统发育分析的方法,分析了包括土壤脲酶细菌在内的土壤脲酶相关理化性质,并分析了土壤与其关联材料及其他环境中脲酶活性细菌的种类及系统发育情况.结果表明,土壤脲酶活性细菌、 有机质、 总氮、C/N、 硝酸根离子、 无机氮含量是土壤脲酶活性的关联因素,且土壤有机质、C/N和土壤脲酶活性细菌是直接影响因素.通过细菌分离和16s rRNA测序,土壤中分离出2门5属9种脲酶活性细菌,厚壁菌门是优势类群,土壤关联脲酶活性材料中发现3门8属9种脲酶活性细菌,变形菌门是优势类群.仅粪产碱菌和绿脓杆菌2种细菌在2种材料(即土壤及土壤关联脲酶活性材料)中均被发现.%To explore the feasibility of using urease activity bacteria to control soil urease activity, soil, urease ac-tivity materials related and non-related soil were used as materials, soil physicochemical properties analysis, ure-ase activity bacterial isolation and phylogenetic analysis were used to analyze soil physical and chemical properties including soil urease bacteria and the diversity of soil urease activity bacterial. The results found that the effective facters of soil urease activity were the number of urease-active bacteria, the content of organic matter and total ni-trogen, C / N, et al. Especially, the number of urease-active bacteria, the content of organic matter and C / N were directly effective factor of soil urease-activity. The diversity of urease-active bacteria was studied based on 16S rRNA BLAST and phylogenetic analysis. The results found that there were 9 species of urease-active bacteria in soil, and belong to 5 genus and 2 phylums, the majority of urease-positive species were in the Firmicutes. In urease activity material associated with soil, 9 species of urease-active bacteria were found which belong to 8 genus and 3 phylums, the majority of urease-positive species were in the Proteobacteria. Only Alcaligenes faecalis and Pseudomonas aeruginosa were found in both two materials.
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