生姜醇提取物诱导TM4细胞凋亡的研究

摘要

The application of Zingiber officinale extraction in alcohol （CCM） has aroused received wildly attention, and espe- cially in the field of medicine the application of it has been attached great importance. The present study was mainly to inves- tigate the effect of Zingiber offieinale extraction in alcohol on cultured T1VI4 cells, and the morphological observation, MTT method and Tunnel method were used to detect the action of Zingiber officinale extraction in alcohol on TM4 cells cultured in DMEM medium and treated with 5 of different concentration. The results indicated that Zingiber offieinale extraction in alco- hol inhibits the growth and proliferation of TM4 cells, and induces the apoptosis of cultured TM4 cells. These effects is dose --dependent. When the euhured TM4 cells was treated with 40μM/mL Zingiber officinale extraction in alcohol, the inhibitory rate of TM4 cells from MTT method and apoptosis index of TM4 cells from TENNEL method all have the significantly differ- ences compared with the data of control. These results suggested that the Zingiber officinale extraction in alcohol has the ap- plication prospects in the prevention and treatment of testicular cancer.%生姜醇提取物的应用已引起了广泛的关注，特别是在医药方面得到了人们的高度重视。本研究主要探讨生姜醇提取物对支持细胞系TM。体外生长的影响。通过采用形态学观察、MTT法和TUN—NEL法检测5个不同浓度的生姜醇提取物对小鼠支持细胞株TM4细胞生长和增殖的作用。结果表明，生姜醇提取物抑制TM4细胞的增殖并诱导其细胞凋亡，这种作用呈剂量依赖效应，当用40μM／mL的生姜醇提取物处理培养的TM4细胞时，MTT法测得的细胞生长抑制率和TENNEL法获得的细胞凋亡指数与对照组相比均有显著差异。这些结果说明，生姜醇提取物在预防和治疗睾丸癌方面有应用价值。