纳米银溶胶的制备及其与荧光素相互作用的研究

摘要

Under the irradiation of sunlight blue and red colours of nano-silver sols were prepared by controlling the intensity and the irradiation time using silver nitrate and trisodium citrate as the reactants and polyvinylpyrrolidone as the capping agent. The ultraviolet-visible absorption (UV-Vis) spectra were monitored and compared during the evolution of the two colour nano-silver sols. And the interactions of fluorescein with the nano-silver sols were investigated by UV-Vis spectrometry and fluorescence photometry, respectively. It was found that the absorbance and fluorescence intensity of fluorescein was enhanced and the peak position of the UV-Vis spectrum had a red shift compared with that of fluorescein itself on adding nano-silver sols into the FL solutions.%以太阳为光源，以硝酸银和柠檬酸三钠为反应试剂、聚乙烯吡咯烷酮为保护剂，通过控制光照时间和光照强度，制备了蓝色和红色纳米银溶胶，比较了两种不同颜色纳米银溶胶形成过程的U V-Vi s吸收光谱；采用 U V-Vi s 分光光度法和荧光法研究了荧光素和两种颜色纳米银溶胶的相互作用。实验发现，纳米银的加入使得荧光素的吸收峰红移，并使得荧光素荧光增强。